FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486853
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG151503.1118222543560377No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT14710.30214458984539483No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC14570.2992689785212374No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13850.2844801202827137No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA11160.22922730269711802No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC11070.22737869541730257No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC10850.22285987762219808No Hit
GTCCTGGGCCCAGTCTGGCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCACCATCTCCTG10420.2140276428408575No Hit
CTCATATACAGTCAATGAGTCGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTTGGTCAGCCCAAGGCCAACCC10320.21197363475217368No Hit
GTATATGAGTAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA10050.20642781291272724No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG9030.18547693040815194No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT8840.18157431503965263No Hit
GATTATTACTGCTACTCATATACAGTCAATGAGTCGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTTGGTCAG8500.17459068753812754No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG8320.17089347297849658No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8070.16575845275678694No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC7990.16411524628583987No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCATTGGAACCAGCAGTGACGTT7720.15856942444639346No Hit
GCCTTGGGCTGACCAAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCGA7540.15487220988676254No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGGCCTGACTCAGCCTGCCTCCGTGTCTGGATCTCCTGGACAGTCGATCAC7490.15384520584242062No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT7420.1524074001803419No Hit
GACCAAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCGACTCATTGACTGTATATGAGTAGCAGTAATAATCAG7080.1454237726788168No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTACTCATATACAGTCAATGAGTCGGTCTTCGGAA6960.1429589629723962No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG6950.1427535621635278No Hit
TCCTTGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG6750.1386455459861601No Hit
GTCGATCACCATCTCCTGCATTGGAACCAGCAGTGACGTTGGTGATAATAAATATGTCTCCTGGTACCAACAACA6550.13453752980879238No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC6470.13289432333784532No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG6330.13001871201368792No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG6210.1275539023072673No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCAAGGACGGTGACCTTGGTCCCAGTTCCGAAGACCGACTCATTGA6180.12693769988066214No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC6110.12549989421858343No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5760.11831086590818995No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC5460.11214884164213838No Hit
CATATACAGTCAATGAGTCGGTCTTCGGAACTGGGACCAAGGTCACCGTCCTTGGTCAGCCCAAGGCCAACCCCA5280.10845162708250745No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA5270.10824622627363906No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG5120.10516521414061328No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGGCCTGACTCAGC5100.10475441252287651No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT4900.1006463963455088No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGG200.005458720555.8036963
CCTAACG200.0083738850.0817072
GGTATCA5500.045.570961
TGGTATC1450.039.144322
GTATCAA14550.036.9787751
GTGGTAT1450.036.8758131
TATCAAC16400.033.1842232
TCAACGC17500.030.894874
TATTATG500.00447642230.86439567
ATCAACG17750.030.6509023
CAACGCA17750.030.6477535
AACGCAG18050.030.323276
ATGACGG450.00542846529.6719520
CTTGTAC450.00543119229.6689033
CCGGAGG2252.6238922E-829.19052370
GGATATT2750.029.1654171
CGCAGAG19050.028.73158
TTGTACT704.823279E-528.6063634
ACGCAGA19350.028.4585257
CAGAGTA19400.028.38517810
Produced by FastQC (version 0.11.5)