FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1450447
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42560.2934267849842152No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40290.27777643719487854No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG28220.1945607112841765No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27930.19256132764589123No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27460.18932094726660126No Hit
GTTACAATACCCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA27310.18828678331576404No Hit
ACTTACTACTGTCAACAGAGTTACAATACCCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA25360.1748426519548801No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24370.16801716987935444No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24020.16560412066073424No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23400.16132957633060704No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGCCAGAGCATTAGTAG22730.1567103106835341No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22090.15229787782662862No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG21320.1469891695456642No Hit
GTAGTAAGTTGCATAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTCGCAGATCCACTGCCA21110.1455413400144921No Hit
GTATTGTAACTCTGTTGACAGTAGTAAGTTGCATAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGT21080.14533450722432464No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20700.142714625215537No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20510.1414046842111432No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC20340.14023263173352768No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20300.13995685467997107No Hit
GAGTTACAATACCCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT20120.1387158579389664No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG19910.13726802840779428No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19840.13678541856407023No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGCCAGA19670.13561336608645475No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19400.13375187097494773No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18060.12451333968080186No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC17960.12382389704691038No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17490.1205835166676204No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17030.11741208055171957No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16800.11582636249376915No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGCCAGAGCATTAGTAGCTACTTAAATTGGTATCAGCAGAAA16770.11561952970360172No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16490.11368909032870556No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT16080.11086237552975049No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15740.10851827057451943No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14980.10327850655694416No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14770.10183067702577206No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14680.10121017865526971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT1850.053.68515437
CGCGTAT200.007469295551.55634739
ATCGCGT200.007576328751.37114337
TCGTATG2000.049.9251740
CTAGCGC3600.048.06292328
ACTAGCG3750.047.0158927
TAGCGCT3650.046.531329
CGTATGC2450.046.44831541
ATGCCGT2550.046.21772844
CTTATAC4250.044.6187251
GTATGCC2950.042.16107642
CGCTCAT3600.041.6343532
GCGCTCA3850.041.54484631
ACGAGAC4250.041.43881622
TCTCCGA4250.040.62230711
CCCACGA4350.040.47643319
CCTAACG858.185452E-1139.8354032
CACGAGA4600.039.7529121
GAGACTA4450.039.5818624
TATACAC5050.038.8888173
Produced by FastQC (version 0.11.5)