FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1450447
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43540.30018332279635174No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34380.2370303775318919No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31630.21807070509987614No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG28550.1968358719760184No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27860.1920787178021672No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25470.17560103885216075No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24850.17132649452203355No Hit
GTTACAATACCCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA24240.16712089445529552No Hit
ACTTACTACTGTCAACAGAGTTACAATACCCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA24070.16594884197767998No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23940.16505256655362105No Hit
GTAGTAAGTTGCATAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTCGCAGATCCACTGCCA22200.15305626472390924No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGCCAGAGCATTAGTAG21380.1474028351259991No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG20650.14236990389859128No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGCCAGAGCATTAGTAGCTACTTAAATTGGTATCAGCAGAAA20300.13995685467997107No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC20140.13885374646574472No Hit
GTATTGTAACTCTGTTGACAGTAGTAAGTTGCATAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGT20130.13878480220235553No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19980.13775063825151834No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19920.13733697267118344No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19680.13568231034984388No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCAAGCCAGA19570.13492392345256327No Hit
GAGTTACAATACCCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT19140.13195932012682987No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19070.1314767102831058No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19060.13140776601971668No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18690.12885682827431819No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18000.12409967410046696No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17950.12375495278352122No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17730.12223817898895994No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17630.12154873635506847No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17400.11996301829711807No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16880.11637791660088234No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16760.11555058544021257No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC16570.11424064443581874No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT15740.10851827057451943No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15590.10748410662368223No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15520.10700149677995818No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15250.10514000166845117No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14610.10072756881154567No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14570.10045179175798909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT1207.4578566E-1153.10247870
GTGGTAT5250.039.2795221
GTACGTA1309.094947E-1238.04137469
GTATCAA44150.036.6775631
GGTACGT1303.6379788E-1133.678268
TATCAAC49500.032.5588532
ATCAACG49950.032.3985823
TCAACGC50450.032.2069784
TGGTACG1306.91216E-1131.94548667
CAACGCA51400.031.5481685
AACGCAG52100.031.5122626
CGATTCG450.004997530530.18218429
CGCAGAG55450.029.7912278
ACGCAGA55550.029.6767857
TAACGCT500.006269380528.81383960
AGAGTAC57250.028.73555211
GCAGAGT58200.028.4416079
ACGGGAT1655.456968E-1226.62963116
CAGAGTA65350.025.48486910
AATGGTA6050.024.57635570
Produced by FastQC (version 0.11.5)