FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605540
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50690.3157193218481009No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45100.28090237552474556No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGCTACCA38120.23742790587590468No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37460.23331713940481086No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36330.22627900893157443No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34380.21413356253970625No Hit
GTATTATAGTGGCCTCATCAATTATAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGCAGACACGTCCAAGAG33350.20771827547118102No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30260.18847241426560535No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30030.1870398744347696No Hit
GTCTCCAGCCACCCTGCCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG29900.18623017800864508No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28230.1758286931499682No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27200.169413406081443No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26150.16287355033197554No Hit
GCCTAGAGCCTGAAGATGCTGGAGTTTATTACTGTCAGCTGCGTAGCAAC25520.15894963688229508No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACGTAGCCTGGTACCAACAGAAACCTGGCCGGGCTCCCAGGCT25490.1587627838608817No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25260.15733024403004597No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATGCTGGAGTTTATTACTGTCAGCTGCGTAGCAACTGG25250.15726795968957485No Hit
GTAATAAATGGCCGTGTCCGCCACACTCACAGAGGTCAGAATCAGGGAGA25080.15620912590156583No Hit
CTATAATACATATGGCCAATCCATTCGAGTCCCTTCCCTGGGGACTGTCG23870.14867272070456045No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23810.14829901466173373No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23410.14580764104288899No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT23380.14562078802147566No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22900.14263113967886193No Hit
GTCCCAGACCCACGGCCGCTGAACCTGGCTGGGATGCCAGCGGCCCTGTG22690.14132316852896845No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22590.14070032512425726No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22180.1381466671649414No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21760.1355307248651544No Hit
GTAATAAACTCCAGCATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACGGCCG21610.13459645975808762No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21520.13403590069384755No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATG20960.1305479776274649No Hit
GTTTATTACTGTCAGCTGCGTAGCAACTGGCCTTCCACGATCACCTTCGGCCAAGGGACACGACTGGAGATCAAA20260.1261880737944866No Hit
GTGTTGACACAGTCTCCAGCCACCCTGCCTTTGTCTCCAGGGGAAAGAGC19150.11927451200219241No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19140.11921222766172129No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC18890.11765511914994331No Hit
GTCTCTGGTGACTCAATCAGTGGTTACTACTGGAACTGGATCCGACAGTC18710.11653400102146318No Hit
GAGTGAAGTCTGTCCCAGACCCACGGCCGCTGAACCTGGCTGGGATGCCA18690.11640943234052094No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAG18340.11422948042403179No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18310.11404262740261843No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18020.112236381528956No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17930.11167582246471591No Hit
GTCAGAGTGTTAGCAGCTACGTAGCCTGGTACCAACAGAAACCTGGCCGGGCTCCCAGGCTCCTCATCTATGATG17910.11155125378377367No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT17770.11067927301717802No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17540.1092467331863423No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17500.10899759582445781No Hit
CCACAGGGCCGCTGGCATCCCAGCCAGGTTCAGCGGCCGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG17490.10893531148398669No Hit
GGACACGACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC17310.10781419335550656No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17120.1066307908865553No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16960.10563424143901741No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16960.10563424143901741No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16700.10401484858676832No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16160.1006514942013279No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGTGGAAGGCCAGTTGCTACGCAGCTGACAGTAATAAACTCCAGCATCTT16110.1003400724989723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT554.1836756E-1156.3012339
TCGCGTA552.7102942E-949.9446538
GGTATCA17950.047.4863241
TCGACGT6450.046.43918629
GACGTCA6500.044.58616631
CGACGTC6600.044.38833630
CTCGACG6750.044.34614628
ATCGCGT704.7111826E-1044.0850137
TCGTATG2750.043.87015540
ACGTCAT6150.042.17228332
CGTATGC3200.042.06279441
AGACTCG7400.041.78164325
CGAGACT7550.040.9336823
CTTATAC7650.040.3960881
CGTTTGC602.9028706E-740.2652441
CACGAGA7750.039.87360421
CCCACGA7750.039.86987719
TATACAC7900.039.5426643
ATCTCCG7750.039.42437710
GCCGTCT3100.039.2835146
Produced by FastQC (version 0.11.5)