FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605540
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64680.40285511416719605No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52380.32624537538772No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40830.25430696214357784No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38300.23854902400438482No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGCTACCA37700.2348119635761177No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35310.21992600620352032No Hit
GTATTATAGTGGCCTCATCAATTATAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGCAGACACGTCCAAGAG31780.1979396340172154No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31070.19351744584376596No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30350.18903297332984542No Hit
GTCTCCAGCCACCCTGCCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG30240.1883478455846631No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28580.1780086450664574No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28200.17564184012855488No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25690.16000847067030408No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACGTAGCCTGGTACCAACAGAAACCTGGCCGGGCTCCCAGGCT25430.15838907781805497No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24610.15328176189942325No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24500.15259663415424096No Hit
GTAATAAATGGCCGTGTCCGCCACACTCACAGAGGTCAGAATCAGGGAGA23950.1491709954283294No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATGCTGGAGTTTATTACTGTCAGCTGCGTAGCAACTGG23900.1488595737259738No Hit
GCCTAGAGCCTGAAGATGCTGGAGTTTATTACTGTCAGCTGCGTAGCAAC23730.1478007399379648No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23440.14599449406430234No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22710.1414477372099107No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22690.14132316852896845No Hit
CTATAATACATATGGCCAATCCATTCGAGTCCCTTCCCTGGGGACTGTCG22530.14032661908143057No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22180.1381466671649414No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21680.13503245014138546No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATG20860.1299251342227537No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20710.12899086911568694No Hit
GTCCCAGACCCACGGCCGCTGAACCTGGCTGGGATGCCAGCGGCCCTGTG20090.12512924000647757No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19980.12444411226129526No Hit
GTAATAAACTCCAGCATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACGGCCG19840.12357213149469959No Hit
GTTTATTACTGTCAGCTGCGTAGCAACTGGCCTTCCACGATCACCTTCGGCCAAGGGACACGACTGGAGATCAAA19720.12282471940904618No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC19680.12257558204716171No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19640.12232644468527722No Hit
GTGTTGACACAGTCTCCAGCCACCCTGCCTTTGTCTCCAGGGGAAAGAGC19310.12027106144973032No Hit
GTCTCTGGTGACTCAATCAGTGGTTACTACTGGAACTGGATCCGACAGTC19130.11914994332125016No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19020.11846481557606786No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18430.11479003948827186No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAG17770.11067927301717802No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17760.1106169886767069No Hit
GGACACGACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC17660.10999414527199572No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17520.10912216450540005No Hit
GAGTGAAGTCTGTCCCAGACCCACGGCCGCTGAACCTGGCTGGGATGCCA17360.10812561505786214No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17220.10725363429126648No Hit
CCACAGGGCCGCTGGCATCCCAGCCAGGTTCAGCGGCCGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG17170.1069422125889109No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16600.10339200518205713No Hit
GTCAGAGTGTTAGCAGCTACGTAGCCTGGTACCAACAGAAACCTGGCCGGGCTCCCAGGCTCCTCATCTATGATG16590.10332972084158602No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16490.10270687743687483No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16300.10152347496792356No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGTGGAAGGCCAGTTGCTACGCAGCTGACAGTAATAAACTCCAGCATCTT16080.10015321947755897No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGAC200.00788000350.8632553
GTGGTAT4500.045.993971
ACTGCGT306.874346E-445.2103738
GGTATCA18250.045.1781
TGGTATC4900.041.5210232
GTATCAA50700.040.153821
AGCGTCC350.001468614638.74691812
GGTGCGT400.002814202333.93844221
TATCAAC59700.033.624842
TGTTAGA19350.033.56535770
ATCAACG60350.033.375063
TCAACGC61050.032.936844
GACAGGT3000.032.798981
CAACGCA61700.032.588845
AACGCAG62550.032.417036
CGCAGAG66200.030.4760278
ACGCAGA66450.030.4123957
GCAGAGT68050.029.7969939
AGAGTAC68250.029.60938611
AGGTATC1551.8189894E-1228.4396692
Produced by FastQC (version 0.11.5)