FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1142646
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTTACGCACCGTTCACTTTTGGCCGGGGGA35040.30665665481697746No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31030.27156267120350486No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26320.23034255578718169No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA23540.20601306091300367No Hit
GTATCCAAGAGGGCCGCTGGCATCCCGGACAGGTTCAGTGGCAGTGGGTC23020.20146222014517184No Hit
GTGCGTAACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT22440.19638628236566705No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22420.19621125002844275No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22260.19481099133064833No Hit
CATCTACACTGTATCCAAGAGGGCCGCTGGCATCCCGGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA21710.18999760205698002No Hit
GTTACGCACCGTTCACTTTTGGCCGGGGGACCAAGGTTGAGATCAAACGA20850.18247121155633503No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19700.17240685216593765No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18970.16601817185725062No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18240.1596294915485636No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18050.15796668434493272No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT16530.14466422671588577No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16430.14378906502976424No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15720.13757541705830154No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA15580.1363501906977314No Hit
GTGTATTACTGTCAGCAGTATGGTTACGCACCGTTCACTTTTGGCCGGGGGACCAAGGTTGAGATCAAACGAA15520.1358250936860585No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15160.13267451161602106No Hit
GTGTAGATGAGGAGTCTGGGAGCCTGGCCAGGCTTCTGTTGGTACCAGGC14740.12899883253431071No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14530.12716099299345554No Hit
CTTGGATACAGTGTAGATGAGGAGTCTGGGAGCCTGGCCAGGCTTCTGTT14460.1265483798131705No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14000.12252263605701152No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13970.1222600875511751No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13760.1204222480103199No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13140.11499624555636653No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12980.11359598685857211No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12600.11027037245131038No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12510.10948272693380103No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT12310.10773240356155799No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12020.10519443467180561No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT11510.10073111007258592No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)