FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1142646
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTTACGCACCGTTCACTTTTGGCCGGGGGA31530.2759384796341124No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29490.2580851812372336No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24430.21380199991948512No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23640.2068882225991252No Hit
GTATCCAAGAGGGCCGCTGGCATCCCGGACAGGTTCAGTGGCAGTGGGTC23250.2034750920232513No Hit
GTGCGTAACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT22610.1978740572320736No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22110.19349824880146607No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21560.18868485952779776No Hit
CATCTACACTGTATCCAAGAGGGCCGCTGGCATCCCGGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA21330.18667198764971826No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20900.18290879239939578No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19700.17240685216593765No Hit
GTTACGCACCGTTCACTTTTGGCCGGGGGACCAAGGTTGAGATCAAACGA19590.17144417431120398No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17420.15245316572236722No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA17400.1522781333851429No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17400.1522781333851429No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17040.14912755131510547No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT16410.14361403269253994No Hit
GTGTATTACTGTCAGCAGTATGGTTACGCACCGTTCACTTTTGGCCGGGGGACCAAGGTTGAGATCAAACGAA15770.13801299790136226No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15590.13643770686634354No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15580.1363501906977314No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15260.13354967330214257No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13310.1164840204227731No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT13280.11622147191693666No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13190.1154338263994273No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12910.11298337367828706No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12860.11254579283522632No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12610.11035788861992253No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12450.10895762992212811No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12430.1087825975849038No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12370.1082575005732309No Hit
GTGTAGATGAGGAGTCTGGGAGCCTGGCCAGGCTTCTGTTGGTACCAGGC12350.1080824682360066No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT12210.10685724187543648No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12020.10519443467180561No Hit
CTCCAGGCACCTTGTCTCTGTCTCCAGGGGAAGGACCCACCCTCTCCTGC11500.10064359390397376No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG11430.1000309807236887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15350.042.036671
GTATCAA43750.036.6791151
TACGTAT1608.260031E-833.90218770
CTAACGC601.554216E-533.7700843
ATCAACG46550.033.298543
TCAACGC47100.032.906824
TATCAAC47400.032.845342
CAACGCA47950.032.3967445
AATGGTA8650.032.13848570
AACGCAG48500.031.9597286
CGCAGAG49550.031.0098658
CCTAACG553.6910624E-430.701422
ACGCAGA50450.030.657487
AGAGTAC49950.030.55871411
GCAGAGT51100.030.0692569
GAGTTTA5100.029.8088781
AAGTGCG1152.1482265E-929.35757814
CAGAGTA53600.028.66676710
TTCGATC605.521118E-428.66123239
TCCGAGC1054.3491855E-728.05468465
Produced by FastQC (version 0.11.5)