FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026394
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24220.23597176133141853No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22380.21804492232027858No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19240.18745238183387666No Hit
GATCTATGCTGCGTCTACTTTACAAAGTGGGGTCCCACCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCA17960.17498153730438798No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17810.17352011021108854No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16460.16036726637139345No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC15590.1518909892302566No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15010.14624013780283204No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14970.14585042391128553No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGGGCATTAATGGTGATTTAGGCTGGTATCAGCAGAAA14260.13893300233633477No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13920.13562043425818937No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGCC13860.13503586342086957No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13810.13454872105643642No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13780.13425643563777653No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13570.1322104377071573No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13520.13172329534272414No Hit
ACTTATTACTGTCTGCAAGATGACGATTATCCACTCACCTTCGGCCCTGGGACCAAAGTGGATGTCAAACGAA13330.12987215435787816No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13260.12919015504767176No Hit
GATTTCACTCTCACCATCAGCAGCCTGCACCCTGAAGATTTTGCGACTTATTACTGTCTGCAAGATGACGATTAT13200.12860558421035195No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12600.12275987583715414No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12240.11925245081323547No Hit
ATACATAGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG12060.11749873830127612No Hit
CCTTCGGCCCTGGGACCAAAGTGGATGTCAAACGAACTGTGGCTGCACCA11990.11681673899106972No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT11920.1161347396808633No Hit
GTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCCCAGGGCCGAAGGTG11780.11477074106045049No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11560.11262731465694459No Hit
GTAATAAGTCGCAAAATCTTCAGGGTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTGCCAGATCCACTGCCG11490.11194531534673818No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11460.1116530299280783No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11440.11145817298230504No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11370.11077617367209863No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11270.10980188894323233No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11230.10941217505168581No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCC11020.10736617712106657No Hit
CAGTAATAAGTCGCAAAATCTTCAGGGTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTGCCAGATCCACTGCCG10990.10707389170240668No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10580.10307932431405484No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10560.10288446736828158No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT10470.1020076111123019No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10350.10083846943766235No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA405.40337E-542.5640141
CTAACGC405.404924E-542.5619433
ACTAGCG2650.041.1886927
CCTAACG504.205611E-640.8594632
ACGAGAC2800.040.14156722
CGTATGC2100.039.5125441
GTATCAA32200.038.9156721
TAGCGCT2750.038.49191729
CTAGCGC2900.037.6526528
GACTAGC2950.036.9783526
TCTCCGA3050.036.83332411
GTGGTAT2800.036.4834441
GAGACTA3100.036.2763524
CGAGACT3150.035.6900923
ATGCCGT2150.035.51632344
TCGTATG2050.035.36248840
CACGAGA3100.035.15477821
AGACTAG3200.034.0844325
CCCACGA3250.033.52571519
TGCACCG2350.033.323476
Produced by FastQC (version 0.11.5)