FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026394
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26130.2545805996527649No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24940.24298661137925592No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19200.18706266794233015No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18730.18248352971665852No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17510.17059725602448964No Hit
GATCTATGCTGCGTCTACTTTACAAAGTGGGGTCCCACCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCA16600.16173126499180626No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14810.14429156834509946No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14720.14341471208911977No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13700.1334770078546835No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13650.13298986549025033No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13490.13143100992406426No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12860.12529301613220656No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12650.12324701820158732No Hit
ACTTATTACTGTCTGCAAGATGACGATTATCCACTCACCTTCGGCCCTGGGACCAAAGTGGATGTCAAACGAA12650.12324701820158732No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12210.11896016539457559No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGCC12180.11866787997591567No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12130.11818073761148253No Hit
GTAATAAGTCGCAAAATCTTCAGGGTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTGCCAGATCCACTGCCG12070.11759616677416275No Hit
GATTTCACTCTCACCATCAGCAGCCTGCACCCTGAAGATTTTGCGACTTATTACTGTCTGCAAGATGACGATTAT12040.11730388135550286No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGGGCATTAATGGTGATTTAGGCTGGTATCAGCAGAAA11780.11477074106045049No Hit
GTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCCCAGGGCCGAAGGTG11750.11447845564179057No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11660.11360159938581091No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11640.11340674244003765No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11610.11311445702137776No Hit
ATACATAGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG11380.11087360214498526No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11270.10980188894323233No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10980.10697646322952005No Hit
CAGTAATAAGTCGCAAAATCTTCAGGGTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTGCCAGATCCACTGCCG10880.10600217850065374No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10820.10541760766333397No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT10730.1045407514073543No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10650.10376132362426124No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACATCCACTTTGGTCC10640.10366389515137461No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10550.10278703889539495No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10500.10229989653096179No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10400.1013256118020955No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)