FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1353432
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC32880.24293795329207524No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32120.23732259914055528No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28310.20917194214411955No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT25540.1887054539866059No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22410.16557906123100385No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21550.15922484469112597No Hit
GTATCACTGTCAGCAGTATGGTGACTCACCGATCACCTTCGGCCAAGGGA20310.1500629510754881No Hit
GTATGGTGACTCACCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT19230.14208323728122285No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19010.14045774002683548No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACTA18480.13654176936853865No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG18370.13572902074134496No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT18200.13447295468113654No Hit
GTGTATTACTGTGCGAGAGTGTATACTTCTTTTTCTAGAGTGGTTATCCG17820.13166527760537655No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17790.13144361888886918No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17110.12641935464803553No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16960.12531106106549866No Hit
GTGTATCACTGTCAGCAGTATGGTGACTCACCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA16760.12383333628878289No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGGCTGGGACAGACTTCA16460.1216167491237092No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCGCCGACTACTTGGCCTGGTACC16320.12058234178000816No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT16060.11866129957027764No Hit
CAGTGATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGCCCCACTGCCA15540.1148192151508166No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14710.10868665732744608No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14030.10366239308661239No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC13980.10329296189243346No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13940.1029974169370903No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13870.10248021326523978No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13720.10137191968270294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA451.973658E-645.61120638
CTTATAC8100.043.6813471
ACGAGAC7800.043.2392922
CGAGACT7850.042.97817623
CTAACGC706.148184E-1042.908113
AGCATCG3500.041.6301734
CCCACGA8400.040.53481319
CACGAGA8450.039.11849221
TCCGAGC9150.037.92932513
TCTCCGA8900.037.86990711
CCTAACG802.2719178E-937.5445982
GAGACTC9100.037.0828124
ATCTCCG9100.037.037610
AGACTCC9000.036.77821725
TTATACA9750.036.6247022
CGAGCCC9450.036.37343615
GACTCCT9150.036.2208826
GCATCGG2600.035.26104435
TACACAT10450.035.129455
GTATCAA42200.034.6447031
Produced by FastQC (version 0.11.5)