FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1609521
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTGCGCGGACTATAGCTGTGTCTTCCTACCAGTATTACTACGGCATGGACGTCTGGGGCCAAGGGAC50280.312391077842414No Hit
CAGTAATACAGAGCCGTGTCTGCGGCGGTCACAGAGGCCAGGTTCAGAGA49680.30866326068438993No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT41710.2591454227686374No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA35770.22224003290419944No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC34190.21242344772140284No Hit
CTCCTGCACTGGTACCACCAATGACGTTGGTGGTTATGAGTATGTCTCCTGGTACCAACAATCCGCAGGCGAAGC33600.20875776084934586No Hit
GTCTCTGGAGCCTCCATCAGCAGTGGTAATTACTACTGGGGCTGGATCCG31330.19465418593482162No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31050.1929145379277437No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG30470.1893109813416538No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC29270.18185534702560574No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC28640.1779411390096805No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTGATGGCTGTATATGAACTGCAGTAGTAAT27090.1683109446847851No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC27040.16800029325494975No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC26910.1671925995373779No Hit
GTAATACAGAGCCGTGTCTGCGGCGGTCACAGAGGCCAGGTTCAGAGAGA26850.16681981782157548No Hit
GACTATAGCTGTGTCTTCCTACCAGTATTACTACGGCATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTC26210.16284347951968317No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT25310.15725175378264714No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC24340.15122511604384162No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG24060.14948546803676374No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23020.14302391829618874No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22860.14202983372071568No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGTACCACCAATGACGTT22440.13942036171009886No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21750.13513337197837122No Hit
GATTACTACTGCAGTTCATATACAGCCATCAGCACTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT21290.13227537882388613No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTACTACTGCAGTTCATAT20960.1302250793869729No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20890.12979016738520344No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG20810.12929312509746688No Hit
GTATATGAACTGCAGTAGTAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA20620.1281126496640926No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC20600.12798838909215846No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTGATGG20540.12761560737635608No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19830.12320435707269431No Hit
CACCTGCACTGTCTCTGGAGCCTCCATCAGCAGTGGTAATTACTACTGGG19520.12127831820771523No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19470.12096766677787989No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTGATGGCTGTATATGAACTG19430.12071914563401161No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG19200.11929014905676906No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18460.11469250789520609No Hit
GTCGATCACCATCTCCTGCACTGGTACCACCAATGACGTTGGTGGTTATGAGTATGTCTCCTGGTACCAACAATC17910.1112753421670174No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT17780.11046764844944551No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG17750.11028125759154432No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG17500.10872800044236765No Hit
GCTATAGTCCGCGCACAGTAATACAGAGCCGTGTCTGCGGCGGTCACAGAGGCCAGGTTCAGAGAGAACTGGTT16790.10431675013870587No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC16740.10400609870887052No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC16450.10220432041582558No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16430.10208005984389144No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC16370.10170727812808904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG502.9050334E-669.7342770
GCGTAAT200.00781593950.96840711
TCGCGTA352.322551E-549.10731538
TCTACGG15500.048.13913770
TCGTATG1250.046.89755240
GGTATCA17150.046.5745241
CGCGTAT306.381332E-445.89840739
CGTATGC1600.045.32961341
CGAGACG3750.042.5975223
ACGAGAC3750.042.59354822
GTATCAA50400.040.5311281
TCTCGTA1450.040.3018738
CGCTCAT3900.040.1563128
GTATACG557.738487E-540.0494369
GACGCTC4000.039.9724426
CACGAGA4050.036.9188321
AGACGCT4350.036.73798425
TATCAAC56750.036.3496362
ATCTCCG4350.035.93286510
GAGACGC4450.035.9012524
Produced by FastQC (version 0.11.5)