FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1609521
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTGCGCGGACTATAGCTGTGTCTTCCTACCAGTATTACTACGGCATGGACGTCTGGGGCCAAGGGAC50240.3121425566985457No Hit
CAGTAATACAGAGCCGTGTCTGCGGCGGTCACAGAGGCCAGGTTCAGAGA47840.29723128806644955No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39340.24442054499444243No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC38990.24224598498559508No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT37220.23124892436942418No Hit
CTCCTGCACTGGTACCACCAATGACGTTGGTGGTTATGAGTATGTCTCCTGGTACCAACAATCCGCAGGCGAAGC34640.21521931058992083No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA34190.21242344772140284No Hit
GTCTCTGGAGCCTCCATCAGCAGTGGTAATTACTACTGGGGCTGGATCCG31560.19608318251206416No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC30730.19092636877679756No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC29550.18359499503268364No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC29320.1821659984554411No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG28990.18011569901852786No Hit
GTAATACAGAGCCGTGTCTGCGGCGGTCACAGAGGCCAGGTTCAGAGAGA27800.17272219498844688No Hit
GACTATAGCTGTGTCTTCCTACCAGTATTACTACGGCATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTC26900.16713046925141084No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG26670.16570147267416827No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTGATGGCTGTATATGAACTGCAGTAGTAAT25900.1609174406547041No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25740.15992335607923103No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC24090.14967185889466494No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC23700.1472487777419493No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG23100.1435209605839253No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT23060.14327243944005702No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGTACCACCAATGACGTT22080.13718367141528442No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21670.13463632969063466No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21510.1336422451151616No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTACTACTGCAGTTCATAT20250.12581382908331112No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19940.12388779021833204No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC19890.1235771387884967No Hit
GATTACTACTGCAGTTCATATACAGCCATCAGCACTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT19830.12320435707269431No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC19590.12171323020948469No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTGATGGCTGTATATGAACTG19450.12084340620594573No Hit
GTATATGAACTGCAGTAGTAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA19320.12003571248837387No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18230.11326351131796354No Hit
CACCTGCACTGTCTCTGGAGCCTCCATCAGCAGTGGTAATTACTACTGGG18210.1131392507460294No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTGATGG18030.1120209055986222No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG17920.11133747245298446No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG17830.11077829987928085No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17770.11040551816347845No Hit
GTCGATCACCATCTCCTGCACTGGTACCACCAATGACGTTGGTGGTTATGAGTATGTCTCCTGGTACCAACAATC17530.10891439130026884No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16640.10338479584919985No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16540.10276349298952918No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16520.10263923241759505No Hit
GCTATAGTCCGCGCACAGTAATACAGAGCCGTGTCTGCGGCGGTCACAGAGGCCAGGTTCAGAGAGAACTGGTT16230.10083745412455009No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC16120.10015402097891235No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAAGC200.00757053651.3813535
TCTACGG17100.046.07091570
GGTATCA20100.040.0614781
TACGGAT11900.039.96021370
GTATCAA50000.039.616241
ACTCGAT400.002392285335.08138751
GTGGTAT6150.034.8048821
TATCAAC56750.034.4027522
GTATAAC701.0977019E-633.9761961
TGGTATC6350.033.1519552
ATCAACG59000.033.140093
TCAACGC59250.033.054474
CAACGCA60800.032.21285
AACGCAG62100.031.7024366
CGTATCA553.1178727E-431.59892845
CGCAGAG65950.030.006138
ACGCAGA65900.029.8228667
AGAGTAC67700.029.18035511
CAGAGTA68450.029.1085710
GCAGAGT68450.028.9598059
Produced by FastQC (version 0.11.5)