FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200451
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA62240.5184718076789474No Hit
CTATTATAGTTCTCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT60070.5003952681117346No Hit
CTATAATAGTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCT57450.4785701373900309No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39840.3318752702109457No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG38950.32446138992761886No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34860.2903908614345775No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA32660.27206441579039875No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG30380.2530715539409772No Hit
GTAATAAAGGGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATACA28030.23349557791196807No Hit
GTATAAAACACTCTGGCTGGACCTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA26670.22216650242283942No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26160.21791809911441617No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26050.21700177683220723No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24350.2028404324707964No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCGCAGACCCGCTGCCA23590.1965094785209892No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT22620.18842918203241948No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG21780.18143181187736943No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20650.172018682978314No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20400.16993613233693006No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC20370.16968622625996396No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT19990.16652074928506036No Hit
GTTTATTACTGTCAGCACTATTATAGTTCTCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA19350.16118941964311748No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACACT19040.15860705684780138No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18420.1534423312571692No Hit
CTCCTAAGCTACTCATTGACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTG18340.1527759150519263No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18230.1518595927697174No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT17560.14627835705080838No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTGTTTTATA17260.14377929628114766No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGCGACAGATTTCACTCTCAC17200.14327948412721553No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC17010.14169674563976373No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16820.14011400715231193No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16820.14011400715231193No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16720.13928098689575835No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16520.13761494638265118No Hit
GTCAATGAGTAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA16350.1361988119465101No Hit
AACCAGGACAGCCTCCTAAGCTACTCATTGACTGGGCATCTACCCGGGAA15760.131283992432844No Hit
ATTCAGTGGCAGCGGGTCTGCGACAGATTTCACTCTCACCATCAGCAGCC15410.12836842153490646No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15410.12836842153490646No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14900.12412001822648322No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14780.12312039391861893No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14740.12278718581599748No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14740.12278718581599748No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14710.12253727973903142No Hit
CTTTATTACTGTGCGAAAAGTGTTAGTGGACTAAATGATGGTTTTGATAT14270.11887199061019567No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC14040.11695604402012244No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13950.11620632578922421No Hit
ACTCATTGACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGCGACAGATTT13890.11570651363529208No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT13850.11537330553267064No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC13830.11520670148135992No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTGTTTTATACAGCTCCAA13830.11520670148135992No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC13820.11512339945570456No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGCAGCTATGCCATGAACTGGG13760.11462358730177241No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT13620.1134573589425974No Hit
ATTATAGTTCTCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA13340.11112490222424738No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA13120.10929225765982953No Hit
GGCCAAAAGTGAACGGAGAACTATAATAGTGCTGACAGTAATAAACTGCC13110.10920895563417417No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13070.10887574753155273No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTCAATGAGTAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA13050.10870914348024201No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12920.10762621714672235No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12760.10629338473623663No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT12640.10529376042837234No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCGCAGACCCGCTGCCACTGAATCGGTCAGGGACC12470.10387762599223126No Hit
GAACTATAATAGTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT12250.10204498142781337No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12250.10204498142781337No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12060.10046224294036159No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCTA200.007794080351.00372728
GGTATCA8100.042.332771
CTACTCA24450.041.1594670
GTGGTAT2550.039.9412271
CGCTATT350.001457416338.8065221
TAGGTGG10950.037.182285
TGGTATC2750.037.019442
GTATCAA19200.036.9560361
ATAGGTG11100.036.3741534
GTCATAG11400.035.439081
AGGTGGT11750.034.9394766
TCATAGG11500.034.8197332
TAAAGCG400.00249675334.7762947
CATAGGT11750.034.6507233
TCAGGTC11750.034.64927712
GGCTTAG601.4987503E-533.9500431
GTCAGGT12000.033.6460911
GTGGTCA12150.033.230718
TGTGTCG17900.031.7244870
GCTCGGG4100.031.55797470
Produced by FastQC (version 0.11.5)