FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098614
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32750.29810288235904514No Hit
GATCTACACTGCATCCAATCTGCAAAGTGGGGTCCCAGCAAGGTTCAGTG32180.29291452684928465No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28340.2579613949940562No Hit
GTGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTG27390.24931413581112202No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24060.21900321678041604No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21840.19879593742661208No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19020.17312723122042867No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18950.17249006475431772No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18380.16730170924455723No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18250.16611840009320833No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17850.16247744885828871No Hit
ACTTACTACTGTCAACAAAGTTACACTTCTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATGAAACGAA17780.16184028239217776No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17660.1607479970217019No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17440.1587454738424961No Hit
AAGTTACACTTCTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATGAAACGAACTGTGGCTGCACCAT17330.1577442122528932No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG16950.15428530857971953No Hit
GTGTAACTTTGTTGACAGTAGTAAGTTGCAAAGTCTTCGGGTTGCAGACTGCTGATGGTGAGACTGAAATCTGTC16380.14909695306995907No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16330.1486418341655941No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15890.1446367878071825No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15690.14281631218972268No Hit
CTATTTACACTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGA15610.14208812194273876No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCGACTATTTACACTGGTATCAGCAGAAA15610.14208812194273876No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14700.1338049578832966No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14570.1326216487319477No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATATCCACTTTGGTCC14460.1316203871423448No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14460.1316203871423448No Hit
GTAGTAAGTTGCAAAGTCTTCGGGTTGCAGACTGCTGATGGTGAGACTGAAATCTGTCCCAGATCCACTGCCA14440.13143833958059883No Hit
GTGCAGCCACAGTTCGTTTCATATCCACTTTGGTCCCAGGGCCGAAAGTG14370.1308011731144879No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14360.1307101493336149No Hit
GTGTAAATAGTCGCTAATGCTCTGACTTGCCCGGCAAGTGATGGTGACTC14340.1305281017718689No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14070.12807045968829817No Hit
GTCTTCGGGTTGCAGACTGCTGATGGTGAGACTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGCTGGGAC13820.1257948651664734No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCAGTCTCACCATCAGCAGTCTGCAACCCGAAGACTTTGCAACTTA13800.12561281760472742No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13530.12315517552115667No Hit
CCCGAAGACTTTGCAACTTACTACTGTCAACAAAGTTACACTTCTCCATT12970.11805784379226916No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCGA12190.11095798888417588No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA12170.1107759413224299No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12140.11050286997981093No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11840.10777215655362121No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11750.10695294252576427No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG11690.10640679984052634No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC11310.10294789616735267No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT11300.1028568723864797No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT11120.10121844433076585No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11000.10012615896028998No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC10990.10003513517941698No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)