FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098614
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTACACTGCATCCAATCTGCAAAGTGGGGTCCCAGCAAGGTTCAGTG35310.3214049702625308No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32540.2961913829607123No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22940.2088085533226411No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22500.20480350696422947No Hit
GTGTAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTG21880.19916003255010403No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21870.19906900876923106No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21050.1916050587376458No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19840.1805911812520139No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19320.17585794464661839No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19210.1748566830570155No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18100.16475304338011348No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG17470.15901854518511507No Hit
CTATTTACACTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGA17260.15710704578678225No Hit
ACTTACTACTGTCAACAAAGTTACACTTCTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATGAAACGAA17050.15519554638844946No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16220.1476405725759912No Hit
AAGTTACACTTCTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATGAAACGAACTGTGGCTGCACCAT16140.14691238232900727No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15700.14290733597059568No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCGACTATTTACACTGGTATCAGCAGAAA15310.13935740851654904No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15160.13799205180345417No Hit
GTGTAAATAGTCGCTAATGCTCTGACTTGCCCGGCAAGTGATGGTGACTC15100.13744590911821622No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14900.1356254335007564No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14610.13298574385543965No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14520.13216652982758276No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCGA14180.12907172127790106No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14170.1289806974970281No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCAGTCTCACCATCAGCAGTCTGCAACCCGAAGACTTTGCAACTTA14120.12852557859266311No Hit
GTAGTAAGTTGCAAAGTCTTCGGGTTGCAGACTGCTGATGGTGAGACTGAAATCTGTCCCAGATCCACTGCCA14110.1284345548117901No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14100.12834353103091714No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13900.12652305541345732No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA13870.12624998407083834No Hit
GTGTAACTTTGTTGACAGTAGTAAGTTGCAAAGTCTTCGGGTTGCAGACTGCTGATGGTGAGACTGAAATCTGTC13550.12333722308290264No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13520.12306415174028366No Hit
GTGCAGCCACAGTTCGTTTCATATCCACTTTGGTCCCAGGGCCGAAAGTG13110.11933217672449103No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12740.11596429683219038No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC12680.11541815414695245No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12640.11505405902346047No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT12480.11359767852949262No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12360.11250539315901673No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATATCCACTTTGGTCC12220.11123106022679484No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12040.10959263217108102No Hit
CCCGAAGACTTTGCAACTTACTACTGTCAACAAAGTTACACTTCTCCATT11970.10895546570497008No Hit
GTCTTCGGGTTGCAGACTGCTGATGGTGAGACTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGCTGGGAC11860.10795420411536719No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11680.10631577605965334No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11590.10549656203179643No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTACACTGCATCCAATCTGCAAAGTGGGGTCCCAG11410.10385813397608258No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG11370.10349403885259063No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC11200.10194663457774979No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)