FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1246853
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32170.2580095648805433No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29390.23571343213674745No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC29240.2345104033915786No Hit
GTATACTAGCACACCACCGGGGTACACTTTTGGCCAGGGGACCAAGCTGG25940.20804377099786422No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAACA24000.19248459922701391No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23620.18943692640591955No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22040.17676502362347446No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT21750.17443916804948137No Hit
CTATTACTGTCAGCAGTATACTAGCACACCACCGGGGTACACTTTTGGCC21080.1690656396543939No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20930.16786261090922505No Hit
GTCTATTACTGTCAGCAGTATACTAGCACACCACCGGGGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA20700.16601796683329953No Hit
CTCCTGCAGGGCCAGTCAGACTGTCAGCAGCACCTTCCTAGCCTGGTACC20240.1623286786814484No Hit
TTCCTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTGGTGCATCCAACAGGGCCA19490.15631353495560424No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18940.1519024295566518No Hit
CTAGTATACTGCTGACAGTAATAGACCGCAAAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC18870.15134101614223971No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18170.14572688199811845No Hit
GTAATAGACCGCAAAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17710.14203759384626735No Hit
CAGTAATAGACCGCAAAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17700.1419573919299228No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17380.13939093060689592No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGACT17230.13818790186172708No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG17060.13682446928386907No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16430.13177174855415996No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT16280.13056871980899112No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT16070.12888447956575474No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT15990.12824286423499803No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15820.12687943165714002No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15660.12559620099562657No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15150.12150590326205256No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15060.12078408601495123No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14730.1181374227755798No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGACTGTCAGCAG14410.11557096145255294No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14400.11549075953620835No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA13990.11220248096608021No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13800.110678644555533No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13690.10979642347574252No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGACTGTCAGCAGCA13320.10682895257099273No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13150.10546551999313471No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12570.10081380884514855No Hit
GTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGA12510.100332597347081No Hit
ATGTAAGTGTGCCCGGATTACTTCCAGGATCATCCGTTCTTCCGAAGATCCTAATGAGGACATTGTGGAGAGAAA12510.100332597347081No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATT200.007342960751.77884341
CGAGACT2450.047.26815423
ACGAGAC2450.047.26246622
TCGTATG2450.046.4348840
ATGCCGT2550.044.83965744
ATCTCGT2150.044.7352137
CGTATGC2500.044.18461241
CTTCGTT650.001080809941.23623370
CCCACGA2900.041.09641319
TAGGCAT2850.040.697729
GACTAGG2850.040.65522826
GGTATCA15250.039.529781
CTATACC1300.039.299434
GATCTCG2450.039.22093636
CCGAAAC1107.2759576E-1238.85012468
CACGAGA3100.038.44811621
CTAGGCA3050.038.00914428
AGACTAG3100.037.36608525
TACGTAT1801.6734703E-1037.22715470
TATACAC3550.036.4595263
Produced by FastQC (version 0.11.5)