FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1246853
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33040.2649871316025225No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC28570.22913687499649116No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28220.22632980792443055No Hit
GTATACTAGCACACCACCGGGGTACACTTTTGGCCAGGGGACCAAGCTGG24640.19761752187306766No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24430.19593328162983126No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23880.19152217623087886No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAACA23130.18550703250503467No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT22530.18069491752435932No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20980.168263620490948No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20690.16593776491695494No Hit
GTCTATTACTGTCAGCAGTATACTAGCACACCACCGGGGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA20140.16152665951800252No Hit
CTATTACTGTCAGCAGTATACTAGCACACCACCGGGGTACACTTTTGGCC18790.150699400811483No Hit
CTCCTGCAGGGCCAGTCAGACTGTCAGCAGCACCTTCCTAGCCTGGTACC18670.14973697781534792No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18130.1454060743327401No Hit
CTAGTATACTGCTGACAGTAATAGACCGCAAAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC18060.14484466091832798No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG17610.14123557468282147No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGACT17510.14043355551937559No Hit
TTCCTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTTTGGTGCATCCAACAGGGCCA17070.13690467120021366No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16980.13618285395311236No Hit
CAGTAATAGACCGCAAAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16220.13008750831092358No Hit
GTAATAGACCGCAAAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16090.12904488339844392No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT16010.1284032680676872No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15700.12591700866100494No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15500.12431297033411316No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGTT15290.1226287300908768No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT14980.12014247068419452No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14560.11677399019772178No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14260.11436793270738412No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14200.11388672120931657No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13940.11180147138435727No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13930.11172126946801265No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13860.11115985605360056No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGACTGTCAGCAG13550.10867359664691828No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA13460.10795177939981698No Hit
GTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGA13200.10586652957485765No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13180.10570612574216849No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGACTGTCAGCAGCA13020.10442289508065505No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12690.10177623184128362No Hit
GTGTAGCACTTGTTTCTGTCATAAGTGTAGCAGGTCTCTGTAGCACTGTC12670.10161582800859444No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12590.10097421267783772No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG301.0026557E-556.5943952
GGTATCA15400.040.375361
GTATCAA42800.037.9463651
GTGGTAT4600.034.7157141
TACGTAT1201.3413892E-534.5005370
AATGGTA11100.034.18971670
CTAACGC400.002807683533.9539153
TATCAAC47600.033.029252
TCAACGC48100.032.611394
ATCAACG48000.032.6099053
CAACGCA49250.031.8511815
AACGCAG49750.031.6675686
TGGTATC4950.030.869672
TATATCG450.004998025530.1812575
CGCAGAG52350.029.9025978
ACGCAGA53200.029.4886577
AGAGTAC54100.029.18638811
GCAGAGT54700.028.8041699
TAGTCAC2200.028.12568538
ATAGCGG1950.027.67821563
Produced by FastQC (version 0.11.5)