FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400234
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT72990.521270016297276No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC60190.42985672394756874No Hit
GAGTTACAGTGCCCTGTACACTTTTGGCCTGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT59520.42507180942613876No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC50600.36136817131993654No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC45890.32773093640063017No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC45740.326659686880907No Hit
CATCTATGCTGCATCCAGTTTGCGGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAGATCTGGGACAGATTTCA42320.30223519783121966No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40940.29237970224976684No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38230.2730257942601022No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG37740.26952637916234No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG37280.2662412139685224No Hit
CTTTTGGCCTGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA34630.24731580578674708No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34200.2442448904968741No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33970.24260230789996529No Hit
GGCCAAAAGTGTACAGGGCACTGTAACTCTGTTGACAGTAGTAAGTTGCA33690.2406026421298154No Hit
GTACACTTTTGGCCTGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG32360.23110422972160366No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32320.23081856318301086No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31780.22696206491200757No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGAAAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGTGG31540.22524806568045055No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG29460.2103934056736231No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29210.2086079898074179No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28490.20346599211274688No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28410.2028946590355612No Hit
AAAGTGTACAGGGCACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATC28390.2027518257662648No Hit
GCATAGATGAGGAGCTTAGGGGCTTTCCCTGGTAAGTGCTGATACCAATT28230.20160915961189343No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28200.20139490970794882No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC28110.20075215999611493No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27160.1939675797045351No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26460.18896841527916047No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25710.18361216768054484No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCAGGCCAAAAGTG25460.1818267518143396No Hit
GTCTGCATCTGTCGGAGAAAGAGTCACCATCACTTGCCGGGCAAGTCAGA23970.1711856732517565No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCAGGCCAAAAGTGTACAGGGCA22820.16297276026721247No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT22770.16261567709397143No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22050.1574736793993004No Hit
ACAGTGCCCTGTACACTTTTGGCCTGGGGACCAAGCTGGAGATCAAACGA21790.15561684689844696No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21720.15511693045590952No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21700.1549740971866131No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT21680.15483126391731666No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21110.1507605157423688No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21050.15033201593447953No Hit
ACTTACTACTGTCAACAGAGTTACAGTGCCCTGTACACTTTTGGCCTGGGGACCAAGCTGGAGATCAAACGAA20770.14833235016432966No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTGCC20350.1453328515091049No Hit
GCTTAGGGGCTTTCCCTGGTAAGTGCTGATACCAATTTAAATAGCCACTA20280.14483293506656744No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCTACTGCCA20170.14404735208543715No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20000.1428332692964176No Hit
CCCCTAAGCTCCTCATCTATGCTGCATCCAGTTTGCGGAGTGGGGTCCCA19050.13604868900483777No Hit
GGGCACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT18950.1353345226583557No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18890.13490602285046643No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18590.13276352381102016No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18260.13040677486762928No Hit
GTACAGGGCACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAG18010.12862135900142405No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17900.12783577602029375No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17790.12705019303916346No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17700.12640744332732956No Hit
GCACTTACCAGGGAAAGCCCCTAAGCTCCTCATCTATGCTGCATCCAGTTTGCGGAGTGGGGTCCCATCAAGGTT17280.12340794467210481No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17130.12233669515238169No Hit
GAGTGAAATCTGTCCCAGATCTACTGCCACTGAACCTTGATGGGACCCCA17000.12140827890195496No Hit
TCTATGCTGCATCCAGTTTGCGGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAGATCTGGGACAGATTTCACT16960.12112261236336212No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16960.12112261236336212No Hit
GCCCTGTACACTTTTGGCCTGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC16600.1185516135160266No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16330.11662336438052495No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16330.11662336438052495No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16310.11648053111122855No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16260.11612344793798751No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC16200.11569494813009826No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16060.11469511524502332No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16050.11462369861037514No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16050.11462369861037514No Hit
GTATCAGCACTTACCAGGGAAAGCCCCTAAGCTCCTCATCTATGCTGCAT16050.11462369861037514No Hit
GAGCATTAGTGGCTATTTAAATTGGTATCAGCACTTACCAGGGAAAGCCCCTAAGCTCCTCATCTATGCTGCATC15830.11305253264811453No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15640.11169561658979855No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15580.1112671167819093No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15570.11119570014726111No Hit
GGTAAGTGCTGATACCAATTTAAATAGCCACTAATGCTCTGACTTGCCCG15540.11098145024331647No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15530.11091003360866826No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15490.11062436707007543No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15410.11005303399288976No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATT15270.10905320110781483No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC15240.10883895120387022No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14850.10605370245259008No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT14550.10391120341314379No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14480.10341128697060635No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14350.10248287072017963No Hit
GTGCTGATACCAATTTAAATAGCCACTAATGCTCTGACTTGCCCGGCAAG14280.10198295427764216No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14240.10169728773904932No Hit
CCATCCTCCCTGTCTGCATCTGTCGGAGAAAGAGTCACCATCACTTGCCG14210.1014830378351047No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC14070.10048320495002977No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT352.2920845E-549.2165539
TCGCGTA352.3023618E-549.17932538
CCTATCG352.3438533E-549.03101334
CGTATGC1600.045.2878841
TCGTATG1300.045.09269740
ATCGCGT405.0959017E-542.99011237
ACTCGAT359.6114766E-442.23475367
ACGAGAC2200.041.91592422
CGAGACG2150.041.3021723
TATCTCG1500.038.94547736
ATGCCGT1800.038.4903844
GCGTATG451.00464364E-438.31405640
TATCGCG451.0253043E-438.1818436
GACGGAC2150.038.14006826
TACGTAT700.001593242538.10703370
GTATCAA31650.035.818651
CCCACGA2400.035.5666219
CACGAGA2600.035.4635221
CGGACTC2350.034.9240628
ATCTCCG2550.034.8059910
Produced by FastQC (version 0.11.5)