FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400234
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT76020.5429092565956832No Hit
GAGTTACAGTGCCCTGTACACTTTTGGCCTGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT54080.3862211601775132No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51340.36665300228390396No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50580.3612253380506401No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC45450.324588604476109No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC45390.32416010466821976No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT42990.3070201123526496No Hit
CATCTATGCTGCATCCAGTTTGCGGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAGATCTGGGACAGATTTCA40310.28788045426692965No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG38340.27381137724123256No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35250.251743637134936No Hit
CTTTTGGCCTGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA34740.24810138876787735No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34370.24545897328589364No Hit
GGCCAAAAGTGTACAGGGCACTGTAACTCTGTTGACAGTAGTAAGTTGCA33830.24160247501489035No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32810.23431797828077308No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32110.22931881385539846No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGAAAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGTGG32100.22924739722075027No Hit
GTACACTTTTGGCCTGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG31790.22703348154665576No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31070.22189148385198476No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT30780.21982040144718668No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29970.21403565404068178No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28790.20560849115219315No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG27890.19918099403385434No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26920.19225358047297808No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26860.19182508066508883No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26610.19003966479888362No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC26550.18961116499099437No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26110.18646883306647316No Hit
AAAGTGTACAGGGCACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATC25910.185040500373509No Hit
GCATAGATGAGGAGCTTAGGGGCTTTCCCTGGTAAGTGCTGATACCAATT25460.1818267518143396No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25270.18046983575602363No Hit
GTCTGCATCTGTCGGAGAAAGAGTCACCATCACTTGCCGGGCAAGTCAGA25060.17897008642841125No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24930.17804167017798453No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCAGGCCAAAAGTG24820.17725608719685423No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23480.16768625815399427No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22850.1631870101711571No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCAGGCCAAAAGTGTACAGGGCA22060.15754509603394862No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT21840.155973930071688No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21700.1549740971866131No Hit
ACTTACTACTGTCAACAGAGTTACAGTGCCCTGTACACTTTTGGCCTGGGGACCAAGCTGGAGATCAAACGAA20780.14840376679897788No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTGCC20740.14811810026038505No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20430.1459041845862906No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20370.14547568477840134No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCTACTGCCA20330.14519001823980848No Hit
ACAGTGCCCTGTACACTTTTGGCCTGGGGACCAAGCTGGAGATCAAACGA20000.1428332692964176No Hit
GGGCACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT19870.14190485304599088No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19790.1413335199688052No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19770.1411906866995088No Hit
CCCCTAAGCTCCTCATCTATGCTGCATCCAGTTTGCGGAGTGGGGTCCCA19470.13904818766006252No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19220.13726277179385732No Hit
GCTTAGGGGCTTTCCCTGGTAAGTGCTGATACCAATTTAAATAGCCACTA19120.13654860544737524No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18520.1322636073684827No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18080.12912127544396151No Hit
GCCCTGTACACTTTTGGCCTGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC18030.12876419227072047No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17910.12790719265494196No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17740.12669310986592242No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17690.12633602669268135No Hit
GCACTTACCAGGGAAAGCCCCTAAGCTCCTCATCTATGCTGCATCCAGTTTGCGGAGTGGGGTCCCATCAAGGTT17480.12483627736506897No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC17170.12262236169097451No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17140.12240811178702987No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17060.12183677870984422No Hit
GTACAGGGCACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAG16970.12119402899801034No Hit
GAGTGAAATCTGTCCCAGATCTACTGCCACTGAACCTTGATGGGACCCCA16750.11962286303574975No Hit
GTATCAGCACTTACCAGGGAAAGCCCCTAAGCTCCTCATCTATGCTGCAT16580.1184087802467302No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16540.11812311370813736No Hit
GAGCATTAGTGGCTATTTAAATTGGTATCAGCACTTACCAGGGAAAGCCCCTAAGCTCCTCATCTATGCTGCATC16430.11733753072700706No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16250.1160520313033393No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15900.11355244909065199No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15770.11262403284022528No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15720.11226694966698424No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15690.1120526997630396No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15650.11176703322444677No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC15500.11069578370472365No Hit
TCTATGCTGCATCCAGTTTGCGGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAGATCTGGGACAGATTTCACT15490.11062436707007543No Hit
GTGCTGATACCAATTTAAATAGCCACTAATGCTCTGACTTGCCCGGCAAG15430.11019586726218618No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15400.10998161735824155No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15340.1095531175503523No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15100.10783911831879528No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATT14960.10683928543372037No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14920.10655361889512754No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14650.1046253697596259No Hit
GGTAAGTGCTGATACCAATTTAAATAGCCACTAATGCTCTGACTTGCCCG14570.10405403668244022No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14540.1038397867784956No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC14310.10219720418158679No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTAGATCTGGGACAGATTTCACT14170.10119737129651185No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14150.10105453802721545No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT659.25175E-442.56055570
AATGGTA8550.037.20937370
GTATCAA35350.036.43931
GTGGTAT4000.034.0774961
TATCAAC39500.031.8241832
GTACGAC553.5080296E-430.9695823
TCTCGCG4250.030.91899170
ATCAACG41250.030.2263093
TCAACGC41450.030.0783164
CGGCCGA500.005421192429.68375267
AACGCAG42650.029.6334936
CAACGCA42650.029.393875
GATTCAC3250.029.359071
ACGCAGA45650.027.6870467
ATGGCAA5300.027.40337470
CGCAGAG45650.027.3139068
CGGGTAT500.00819619427.27466617
GCGACAA500.00820191427.27076716
CCGTTAA500.00820191427.27076716
AGAGTAC45950.027.2097211
Produced by FastQC (version 0.11.5)