FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004692705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969782
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAATAGTGTACGTCCTACTGTAACTCTGTTGACAGTAGTAACTTGCA32980.340076429548084No Hit
CTATTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCA30530.3148130198333234No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28320.29202439311102907No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23510.24242561730368267No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21210.21870894695921353No Hit
GTAGGAGACAGAGTCATCATCACTTGCCGGTCAAGTCAGAGCATTAGCAACTACTTAAATTGGTATCAACAAAAA19370.19973561068363818No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18150.18715546380526757No Hit
GAGTTACAGTAGGACGTACACTATTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCAT17000.17529712863303298No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16960.17488466480095527No Hit
GTATCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCATCATCACTTGCCGGTCAAGTCAGAGCATTAGCAA16920.17447220096887753No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16600.17117249031225573No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16440.16952263498394485No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16300.1680790115716728No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16290.1679758956136534No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15040.1550864008612245No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14510.14962125508619464No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14220.1466308923036311No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14140.14580596463947568No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13870.14302183377295105No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13820.14250625398285388No Hit
GTGTACGTCCTACTGTAACTCTGTTGACAGTAGTAACTTGCAAAATCTTC13800.14230002206681502No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13680.14106263057058185No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13150.13559748479555198No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12810.13209154222289132No Hit
ATAGTGTACGTCCTACTGTAACTCTGTTGACAGTAGTAACTTGCAAAATC12780.13178219434883304No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12660.13054480285259987No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAATAGTG12650.13044168689458044No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT12650.13044168689458044No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG12570.12961675923042498No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTA12400.12786378794409464No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12280.12662639644786147No Hit
CTGTAACTCTGTTGACAGTAGTAACTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC12250.1263170485738032No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT11440.11796465597422925No Hit
AGTCAGAGCATTAGCAACTACTTAAATTGGTATCAACAAAAACCAGGGAAAGTCCCTAAACTCCTGATCTTTG11420.1177584240581904No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11390.1174490761841321No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11350.11703661235205438No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAATAGTGTACGTCCTA11170.11518052510770461No Hit
GTCCCTAAACTCCTGATCTTTGCTGCATCCACTTTGCAAAGTGGCGTCCCATCAAGGTTCACTGGCAGTGGAT10940.1128088580732577No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10930.11270574211523826No Hit
CTCCTGATCTTTGCTGCATCCACTTTGCAAAGTGGCGTCCCATCAAGGTT10870.11208704636712168No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10750.1108496548708885No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT10560.10889045166851932No Hit
GTAGTAACTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGA10330.10651878463407241No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10170.10486892930576151No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9940.10249726227131459No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)