FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969782
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCAATAGTGTACGTCCTACTGTAACTCTGTTGACAGTAGTAACTTGCA31050.3201750496503338No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29000.2990362782563504No Hit
CTATTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCA28850.2974895388860589No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21240.21901829483327181No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20000.20623191603886235No Hit
GTAGGAGACAGAGTCATCATCACTTGCCGGTCAAGTCAGAGCATTAGCAACTACTTAAATTGGTATCAACAAAAA19250.198498219187405No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19150.1974670596072107No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18690.19272372553831688No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17370.17911241907975192No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17100.1763282882132273No Hit
GTATCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCATCATCACTTGCCGGTCAAGTCAGAGCATTAGCAA16870.17395662117878039No Hit
GAGTTACAGTAGGACGTACACTATTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCAT16050.16550111262118702No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15880.1637481413348567No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15550.16034531472021546No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14430.1487963274220392No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14300.1474558199677866No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13770.1419906741927567No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT13630.14054705078048468No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13130.13539125287951312No Hit
ATAGTGTACGTCCTACTGTAACTCTGTTGACAGTAGTAACTTGCAAAATC12940.13343204967714392No Hit
GTGTACGTCCTACTGTAACTCTGTTGACAGTAGTAACTTGCAAAATCTTC12870.13271023797100792No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12410.1279669039021141No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAATAGTG12390.12776067198607524No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12350.1273482081539975No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12310.12693574432191976No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12230.12611081665776433No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12120.12497654111955057No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11730.12095501875679275No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11710.1207487868407539No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTA11680.12043943896669562No Hit
CTCCTGATCTTTGCTGCATCCACTTTGCAAAGTGGCGTCCCATCAAGGTT11460.11817088789026811No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11400.11755219214215154No Hit
CTGTAACTCTGTTGACAGTAGTAACTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC11330.11683038043601551No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11320.11672726447799608No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11290.1164179166039378No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11270.11621168468789894No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT11250.11600545277186006No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG11250.11600545277186006No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAATAGTGTACGTCCTA11000.11342755382137427No Hit
AGTCAGAGCATTAGCAACTACTTAAATTGGTATCAACAAAAACCAGGGAAAGTCCCTAAACTCCTGATCTTTG10990.11332443786335486No Hit
GTCTGCATCTGTAGGAGACAGAGTCATCATCACTTGCCGGTCAAGTCAGA10680.11012784316475248No Hit
GTCCCTAAACTCCTGATCTTTGCTGCATCCACTTTGCAAAGTGGCGTCCCATCAAGGTTCACTGGCAGTGGAT10480.10806552400436387No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT10400.10724059634020842No Hit
GTAGTAACTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGA10350.10672501655011127No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT9930.10239414631329515No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9850.1015692186491397No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9810.10115675481706196No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACCG252.032302E-457.87149462
TCGCTAT502.0704891E-433.8798725
GGGTCGA400.00286427833.8152127
TCAACGC49950.032.5594374
ATCAACG50000.032.526883
GTGGTAT4350.031.8931941
CAACGCA51450.031.7448925
AACGCAG51450.031.6134436
CGGGTAT751.9390409E-631.58693117
ACGGGAC751.9413874E-631.5820416
CGCAGAG53800.030.232568
ACGCAGA54600.029.9753887
ATGGCAA6800.029.82092570
AGAGTAC55900.029.09680711
GCAGAGT56450.028.7534149
CTCGCTA606.035785E-428.2273924
GAGCTAG606.0723606E-428.198251
TACGTAT1405.5353503E-528.03441670
CAGAGTA59000.027.56799510
GTATCAA61150.027.1699261
Produced by FastQC (version 0.11.5)