FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences885477
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATAGATGGCCGTGTCCTCGTCTCGCAGAGTGTTCATTTGAAGATA24540.27713876249750136No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19610.2214625563396904No Hit
CTATTATTGTGCGCGAGCCGGCGGTGGATACACTTCTGGTTACTTCTACT19480.2199944210860361No Hit
GCCCACTGGTGTCCCGGCCAGGTTTAGTGGCAGTGGGTCTGGGGCAGAAT17820.20124746323168188No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17630.1991017270917257No Hit
CATCTACACGGCCCACTGGTGTCCCGGCCAGGTTTAGTGGCAGTGGGTCT15590.1760632969574591No Hit
GCCCAGCACTGGGCTTCCGAGGTGTTTCCATTCGGTGATCAGCACTGAAC15360.1734658268933016No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14540.1642052814471748No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC14300.16149487790196698No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14170.1600267426483127No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13840.15629993777365195No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13810.15596113733050096No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12440.14048925042660623No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12130.1369883125140461No Hit
GTACCATGCCTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT11920.13461670941198925No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11130.12569496440901345No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTTGGCTGAGCTGGGTTTTC11110.1254690974469128No Hit
GTGTAGATGTGTCATACATGAGGAGCCTGGGAGCCTGGCCAGGTTTTCGCTGGTACCAGGCCAACCTGCCACTGA11100.12535616396586247No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11020.12445269611745986No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11010.12433976263640954No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10770.12162935909120168No Hit
GTACTGCTGACAGTAATAAACTGCAAGATCTTCAGACTGCAGGCTGCTGATGGTGAGACTGAATTCTGCCCCAGA10630.12004829035649713No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10630.12004829035649713No Hit
CCCTATCTGTGTCTCCTGGGGAAAGAGTCACCCTCTCTTGTTGGGCCACGCAGAGTGTCAGTGGCAGGTTGGC10610.11982242339439646No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10570.11937068947019515No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10400.11745082029233961No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10350.11688615288708798No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10160.11474041674713177No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10140.1145145497850311No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10110.11417574934188014No Hit
CAGCAGTACCATGCCTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGA10100.11406281586082981No Hit
CCAGTGGGCCGTGTAGATGTGTCATACATGAGGAGCCTGGGAGCCTGGCCAGGTTTTCGCTGGTACCAGGCCAAC9810.11078774491037034No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9780.11044894446721935No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9740.10999721054301805No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9470.10694800655465925No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9080.10254360079369651No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8950.10107546554004225No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8940.10096253205899193No Hit
GAGTGTCAGTGGCAGGTTGGCCTGGTACCAGCGAAAACCTGGCCAGGCTCCCAGGCTCCTCATGTATGACACAT8910.10062373161584094No Hit
ACTCTGGGGAGTCCCTGAGAATCTCCTGTGCAGCCTCTGGACTCAGCGTC8900.10051079813479062No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTTA200.002856158265.7233869
CGACATT200.006276645753.8730462
GGTATCA7050.042.4807431
TCGTATG901.8189894E-1242.10946340
TGTTAGA5150.039.3102470
CGTATGC1100.037.65204641
GTATCAA20950.036.7132641
ATCTCGT1350.035.59491737
ATGCCGT1102.7284841E-1134.661344
ATCAACG23200.032.708943
TATCAAC23400.032.4312062
TCAACGC23600.032.154554
CCGTCTT1109.531504E-1031.66876247
AATCTCG1305.456968E-1231.64913436
AACGCAG24000.031.6186396
CAACGCA24150.031.5631585
ATCTCCG1900.030.44358410
GTGTTAG5400.029.21039269
GCCGTCT1202.4647306E-928.97943746
AGAGTAC26650.028.8543811
Produced by FastQC (version 0.11.5)