FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences885477
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATAGATGGCCGTGTCCTCGTCTCGCAGAGTGTTCATTTGAAGATA22410.2530839310337818No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21420.24190351640979946No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19040.21502534791982175No Hit
CTATTATTGTGCGCGAGCCGGCGGTGGATACACTTCTGGTTACTTCTACT18700.21118560956411067No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15690.17719263176796235No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC14610.1649958158145271No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14540.1642052814471748No Hit
GCCCAGCACTGGGCTTCCGAGGTGTTTCCATTCGGTGATCAGCACTGAAC14480.16352768056087283No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13370.1509920641642866No Hit
GCCCACTGGTGTCCCGGCCAGGTTTAGTGGCAGTGGGTCTGGGGCAGAAT12570.14195738568026048No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12510.1412797847939585No Hit
GTACTGCTGACAGTAATAAACTGCAAGATCTTCAGACTGCAGGCTGCTGATGGTGAGACTGAATTCTGCCCCAGA12280.138682314729801No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11910.1345037759309389No Hit
GTGTAGATGTGTCATACATGAGGAGCCTGGGAGCCTGGCCAGGTTTTCGCTGGTACCAGGCCAACCTGCCACTGA11490.1297605697268252No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11320.12784070054896965No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11310.12772776706791933No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTTGGCTGAGCTGGGTTTTC11180.12625963181426508No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11120.1255820309279631No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11030.12456562959851018No Hit
GTACCATGCCTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT10680.12061295776174875No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10620.1199353568754468No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10540.11903188902704419No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10430.1177896207354906No Hit
CCAGTGGGCCGTGTAGATGTGTCATACATGAGGAGCCTGGGAGCCTGGCCAGGTTTTCGCTGGTACCAGGCCAAC9940.11225588016402457No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9870.1114653457966723No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9810.11078774491037034No Hit
CCCTATCTGTGTCTCCTGGGGAAAGAGTCACCCTCTCTTGTTGGGCCACGCAGAGTGTCAGTGGCAGGTTGGC9730.10988427706196773No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9400.10615747218730696No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9180.10367293560419977No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9070.10243066731264616No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9030.10197893338844487No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8880.10028493117268998No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCG100.007956851139.5082970
TACGTAT1151.9097752E-742.45904570
GGTATCA8900.040.12571
CGGCAAA1304.9965456E-737.55992570
GTGGTAT2850.036.9947551
GTATCAA21250.035.6918681
AATGGTA6050.031.12994870
TCGGCAA1002.18465E-631.10542569
TATCAAC24250.030.6841372
TGGTATC3350.030.426922
TTTAGTC450.004983617530.1981224
TCAACGC24800.030.1372414
ATCAACG24800.030.0036433
ATAGCGG853.1687778E-629.65780663
CAACGCA25100.029.6433585
AACGCAG25500.029.3149096
ACGGGAC605.906747E-428.33154516
ATCGGGA500.007108701428.08076751
GTACGTA1305.435377E-727.34542769
TCGGGAT657.7135017E-427.06707852
Produced by FastQC (version 0.11.5)