FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences746732
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26500.3548796623152617No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25730.3445680645800635No Hit
GTAATAGACAGCTGTGTCCTCGTCTCTCAGGTTGTTCATTTGCAGATACA23520.31497243991150775No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20740.27774355458183125No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18550.24841576362068318No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18200.24372867374104767No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15390.2060980378502595No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15000.2008752805558085No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14200.1901619322594987No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14030.18788534574653287No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13490.1806538356465238No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13280.17784158171874248No Hit
GTATTAGCACCTGGTTAGCCTGGTATCAGGAGAAACCAGGGAAAGCCCCT12670.16967265364280626No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12660.1695387367891024No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12660.1695387367891024No Hit
GTAGGAGACAGCGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCACCTGGTTAGCCTGGTATCAGGAGAAA12340.16525339747057846No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11810.15815580422427325No Hit
GGGTATTAGCACCTGGTTAGCCTGGTATCAGGAGAAACCAGGGAAAGCCCCTAGACTCCTGATCTCCGGTGCATC11000.14730853907425956No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10980.14704070536685185No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10810.14476411885388601No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10540.14114836380388146No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10540.14114836380388146No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10110.13538993909461494No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10020.1341846874112801No Hit
GTCTATTACTGTGCGAAGTTGGGTGGCTATTCCTACCACTACGGTCTGGA10000.13391685370387232No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9780.13097068292238714No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT9760.1307028492149794No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9530.12762276157979033No Hit
ACTTACTACTGTCAACAGACTGACAGTTTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCACACGAA9480.12695317731127098No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9400.12588184248164No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9390.12574792562793613No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8970.12012341777237348No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8740.11704333013718442No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGGAA8720.11677549642977669No Hit
GTGCAGCCACAGTTCGTGTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG8150.10914223576865595No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8080.10820481779272885No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8070.108070900939025No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8070.108070900939025No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8010.10726739981680175No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7930.10619606498717077No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGGAAA7860.10525864701124366No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7790.10432122903531656No Hit
CCCCTAGACTCCTGATCTCCGGTGCATCCAGTTTGCAAAGTGGGGTCCCA7770.1040533953279088No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7740.10365164476679718No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT7690.10298206049827784No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7680.10284814364457395No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7650.10244639308346233No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCG200.00700394252.3993647
TTCGCGC200.007511092351.48113638
TCTGGAG6800.048.4758870
CGAGACT1650.043.07822423
ACGAGAC1750.042.54503622
ATCCTTA350.001267360539.9233247
CCCACGA1950.038.1763319
CTTATAC2050.037.9697951
GCTACGG1107.6243305E-637.45863770
GCCCACG2000.037.2219218
TATAGGT656.343598E-736.4386024
CGAGCCC2150.034.62272315
GTATCAA20050.034.264621
ATTTCGG400.00282265133.9153329
CACGAGA2200.033.84264421
ATTGTCT6300.031.92956464
TATACAC2550.031.84553
TACACAT2550.031.84555
TTATACA2450.031.7642612
AATGGTA3300.031.21552870
Produced by FastQC (version 0.11.5)