FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152938
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27650.23982208930575624No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26510.22993430696186612No Hit
GGGTAGAGATCAGGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG24550.21293426012500238No Hit
GTCCTGTGATGCGTCTGGATTCACCTTCAGTCGTTTTGCCATGCACTGGGCCCGCCAGCGTCCAGGCAAGGGG21770.18882194879516503No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21390.18552602134720167No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21070.18275050349628516No Hit
TCTCTACCCTGGTCCCTCCGCCGAAAGAGAGTGGGAAACTTTCAGACTGCTGACAAGTGTAAGTGGCAACATCTT20660.17919437124979834No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19670.17060761289852533No Hit
GTAATAGACAGCCGTGTCGTCGATTTTCAGGCCACTCATTTGCAGATGTA16590.14389325358345376No Hit
GTCTATTACTGTGTTAGAGATTTGGGGGGTCGATGTTACAAAGGAAATTG15910.13799527815025614No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15890.13782180828457383No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15500.13443914590376932No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14800.12836770060488942No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14210.12325033956726208No Hit
CATCATAAGCGACCTGCAGCCTGAAGATGTTGCCACTTACACTTGTCAGCAGTCTGAAAGTTTCCCACTCTCTTT14070.12203605050748609No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13860.12021461691782212No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13530.11735236413406445No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13380.11605134014144733No Hit
GTGTAAGTGGCAACATCTTCAGGCTGCAGGTCGCTTATGATGAGAGTGAA13030.11301561749200739No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12830.11128091883518454No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12480.1082451961857446No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12380.10737784685733319No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12290.1065972324617629No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12180.10564314820051034No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11900.10321457008095838No Hit
CCCTCACACTGTCCTGTGATGCGTCTGGATTCACCTTCAGTCGTTTTGCC11890.10312783514811724No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11740.10182681115550013No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11700.10147987142413556No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11610.10069925702856529No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG400.007400138351.67376370
CCTAACG507.805647E-847.5886422
GTGGTAT3900.042.720731
CTAACGC656.1188075E-736.6098253
TACGTAT850.003518385332.42275670
CCGTTCA553.5081027E-430.9688926
ATCAACG33800.030.1729343
TCAACGC33950.029.7418064
CAACGCA34400.029.454135
AACGCAG34800.029.019136
TACTGGG8100.028.069770
ACGCAGA36550.027.7227367
CGCAGAG36450.027.7055078
ACGCTAT500.007684333227.63710441
AGAGTAC37650.027.09339711
CAGAGTA37550.026.80334310
GCAGAGT38150.026.4709229
CGTCGAT6600.026.29155218
GTATCAA40800.026.0850321
CGATTTT6650.025.59111621
Produced by FastQC (version 0.11.5)