FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080877
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29230.2704285501495545No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGGCGAAAAGCAC27970.2587713495615135No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC26210.24248827572424986No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24350.2252800272371417No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG23670.21898883961819893No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCATCTTTAGGAAGAACTGGTTCTTGGACGTGTCTACTGA23060.21334527425414732No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG21370.1977098226717749No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20970.19400912407239676No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20900.19336150181750558No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGGCGAAAAGCACATGGTCACCA20700.19151115251781653No Hit
AGCCAGGACAGGCCCCTAGAGTTGTCTTCTATGGTAAAAACAGCCGGCCC20170.18660772687364058No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA19810.1832770981342003No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC17810.1647736051373098No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17070.1579273127284603No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17050.1577422777984914No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16300.15080346792465746No Hit
CTATTATGCAACCTGGTACCAGCAGAAGCCAGGACAGGCCCCTAGAGTTGTCTTCTATGGTAAAAACAGCCGGCC15710.14534493749057478No Hit
GTGTATTACTGTGCGAGAGTTTGGGAGGTTAATAGTAACAGTTGGGGTGTCTTTGACTACTGGGGCCAGGGAACC15460.1430320008659635No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15330.14182927382116559No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT15070.1394238197315698No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14850.13738843550191188No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT14330.13257752732272035No Hit
GACTACTACTGTAACTCCCGGGACAGCAGTGGTGACCATGTGCTTTTCGC14210.13146731774290693No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14190.13128228281293802No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCGCATGCCAAGGAGACAGCCTCAGGAA14030.1298020033731868No Hit
CACCTGCACTGTCTCTGGCGCATCCATCAGTACTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGATGGGG13890.12850675886340446No Hit
CCCCTAGAGTTGTCTTCTATGGTAAAAACAGCCGGCCCTCAGGGATCCCA13830.12795165407349773No Hit
GTCCTGGCTTCTGCTGGTACCAGGTTGCATAATAGTTCCTGAGGCTGTCTCCTTGGCATGCGATCCTGACTGT13600.12582375237885532No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13520.1250836126589797No Hit
GTAGTAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG13280.12286319349935283No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13250.12258564110439948No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12930.11962508222489701No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA12560.11620193602047227No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12480.11546179630059664No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12320.1139815168608454No Hit
GTCTCTGGCGCATCCATCAGTACTTACTACTGGAGCTGGATCCGGCAGCC12250.11333389460595424No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12220.11305634221100089No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT12160.11250123742109416No Hit
CTATAACCCCTCCCTCAGGAGTCGAGTCAGCATGTCAGTAGACACGTCCAAGAACCAGTTCTTCCTAAAGATGAG12030.11129851037629628No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC11780.10898557375168497No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC11660.10787536417187155No Hit
CTACTACTGTAACTCCCGGGACAGCAGTGGTGACCATGTGCTTTTCGCCG11520.10658011966208922No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11500.10639508473212031No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11500.10639508473212031No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11380.10528487515230688No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11200.10361956078258673No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10990.10167669401791324No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC10960.10139914162295986No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA10920.10102907176302207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1150.051.0234340
ACGGACT1550.046.42790227
CGTATGC1100.043.9579641
CTTAACG404.477465E-543.94174658
GGTATCA9700.043.7885481
CGAGACG1700.042.31173723
GACGGAC1800.041.8756226
ACGAGAC1750.041.10473322
CGGACTC1700.040.32674828
TATCTCG1300.039.72966436
CACGAGA1850.038.88285421
TACGTTA459.620895E-538.59566547
GTGGTAT3050.038.1847231
TATACAC2550.037.610433
GCCGTCT1151.8189894E-1236.2225746
GTATCAA26400.036.200191
GTATGCC1350.035.86947342
ATCTCGT1550.035.56294637
TCCTATC1650.035.3967633
CTCCTAT1650.035.38033732
Produced by FastQC (version 0.11.5)