FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080877
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG27200.25164750475771064No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26980.2496121205280527No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGGCGAAAAGCAC26380.24406107262898552No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC25920.23980526923970072No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23590.2182486998983233No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCATCTTTAGGAAGAACTGGTTCTTGGACGTGTCTACTGA22560.20871940100492473No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21710.20085541648124625No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA21340.19743227027682148No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19960.18466486010896707No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGGCGAAAAGCACATGGTCACCA19870.18383220292410699No Hit
AGCCAGGACAGGCCCCTAGAGTTGTCTTCTATGGTAAAAACAGCCGGCCC19820.18336961559918474No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG19120.17689339305027307No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC16840.15579941103381792No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16430.15200619496945536No Hit
GTGTATTACTGTGCGAGAGTTTGGGAGGTTAATAGTAACAGTTGGGGTGTCTTTGACTACTGGGGCCAGGGAACC16270.15052591552970412No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15920.1472878042552483No Hit
CTATTATGCAACCTGGTACCAGCAGAAGCCAGGACAGGCCCCTAGAGTTGTCTTCTATGGTAAAAACAGCCGGCC15700.14525242002559033No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15540.1437721405858391No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15450.142939483400979No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14720.136185708457114No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14480.13396528929748713No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCGCATGCCAAGGAGACAGCCTCAGGAA14330.13257752732272035No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14230.13165235267287584No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14220.13155983520789136No Hit
GACTACTACTGTAACTCCCGGGACAGCAGTGGTGACCATGTGCTTTTCGC14000.12952445097823342No Hit
CCCCTAGAGTTGTCTTCTATGGTAAAAACAGCCGGCCCTCAGGGATCCCA13800.12767410167854437No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA13760.12730403181860656No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT13630.12610130477380868No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13360.12360333321922846No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13340.12341829828925956No Hit
CACCTGCACTGTCTCTGGCGCATCCATCAGTACTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGATGGGG13310.12314074589430621No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT13250.12258564110439948No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13230.12240060617443058No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC13060.12082780926969487No Hit
GTCCTGGCTTCTGCTGGTACCAGGTTGCATAATAGTTCCTGAGGCTGTCTCCTTGGCATGCGATCCTGACTGT13050.12073529180471043No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12820.11860739011006803No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12710.11758969799523905No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12180.11268627235106307No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11980.11083592305137402No Hit
GTAGTAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG11700.10824543403180936No Hit
GTCTCTGGCGCATCCATCAGTACTTACTACTGGAGCTGGATCCGGCAGCC11590.10722774191698038No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA11300.10454473543243126No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11210.1037120782475712No Hit
CTATAACCCCTCCCTCAGGAGTCGAGTCAGCATGTCAGTAGACACGTCCAAGAACCAGTTCTTCCTAAAGATGAG11040.1021392813428355No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCGCATGCCAAGGAGACAGCCTCAGGAACTATTATGCAACCTGGTA10940.10121410669299098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9700.043.437461
TACGAAG658.780264E-443.01338270
CGACGAG451.0480545E-438.03882620
TCGACGA451.0483402E-438.03706719
GTATCAA27350.037.5746541
TCCGAGA6100.036.6671570
TAACGAG501.5913064E-435.43225562
TATATTG800.0024358334.94837670
TTCGACG501.9481627E-434.2333618
TATCAAC30050.034.06092
ATCAACG30750.033.5019723
TCAACGC31350.032.966914
GTGGTAT2500.032.8853381
CAACGCA32050.032.246885
AATAACG552.8525217E-432.080560
AACGCAG32400.031.6872886
AGAGTAC33550.030.49913211
CCGTTAA902.1397318E-730.42261116
ACGCAGA34000.030.298187
AATGGTA5550.030.22562270
Produced by FastQC (version 0.11.5)