FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022109
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCTCACCCTACACTTTTGGCCAGGGGA38700.3786288937872575No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31890.31200194891151534No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28960.2833357303379581No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA28650.28030278571072165No Hit
GTATAGGAGACTACGCACAGAGGTTCCAGGGCAGAGTCATCATTACCGCG27950.2734542010685749No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA21260.20800129927434352No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19690.19264090229124292No Hit
GTATGGTAGCTCACCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT19340.18921660997016954No Hit
CTCCTGCAGGGCCAGTGAGAGTATTGGCAGCAACTACTTAGCCTGGTACC18870.18461827456758526No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18640.18236802532802274No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17930.17542160376241672No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACAGACTGGAGCCTGAAGATT17640.17258433298209877No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTGAGAGTATTGGCAG17540.1716059637475064No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17050.16681195449800365No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA16660.1629963144830933No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16050.15702826215207968No Hit
CATATAGGCTGTGCCCGTGGATTTGTCCGCGGTAATGATGACTCTGCCCT15910.1556585452236503No Hit
GTGTATTACTGTGCGAGAGATCCCTCTTGTAGTAGTTCCACCTGTTTTGG15800.15458233906559868No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA15760.1541909913717617No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15410.15076669905068832No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGAACGCCAGTGGCCCTGCT15400.1506688621272291No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15170.14841861288766658No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15100.1477337544234519No Hit
GTAATACACGGCCGTGTCGTCAGATTTCAGCCAGCTCAGATCCATATAGG15100.1477337544234519No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA14770.14450513594929698No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14470.1415700282455198No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14450.14137435439860133No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTC14200.13892843131212035No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14150.13843924669482413No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14150.13843924669482413No Hit
CTCCTATACCAATCATAGGGATGATCCTTCCCATCCACTCAAGCCCTTGT14120.1381457359244464No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC14020.13716736668985402No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13860.13560197591450618No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13750.13452576975645456No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13600.13305821590456596No Hit
CATCTATGCTGCATCCAGCAGGGCCACTGGCGTTCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA13430.13139498820575887No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12980.1269923266500931No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12740.12464424048707133No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12340.12073076354870176No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTGAGA12090.11828484046222078No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG12070.11808916661530228No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11860.11603459122265826No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11850.115936754299199No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAAGGATCATCCCTATGATTGGT11830.11574108045228054No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11630.11378434198309574No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11510.11261029890158485No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11290.1104578865854816No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11240.10996870196818538No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11110.10869682196321527No Hit
GTGTAGGGTGAGCTACCATACTGCTGACAGTAATACACTGCAAAATCTTC10780.10546820348906036No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC10730.10497901887176417No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10660.10429416040754949No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10640.10409848656063102No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10610.10380497579025329No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10470.10243525886182393No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10440.1021417480914462No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10410.1018482373210685No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC10350.10126121578031305No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10340.10116337885685382No Hit
CAGTAATACACGGCCGTGTCGTCAGATTTCAGCCAGCTCAGATCCATATA10290.10067419423955763No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10260.1003806834691799No Hit
GTATTACTGTGCGAGAGATCCCTCTTGTAGTAGTTCCACCTGTTTTGGGGAGTGGGGCCAGGGAACCCTGGTCAC10230.10008717269880218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT703.7289283E-969.9501670
CTACGCT2050.049.79279728
TCGTATG352.3014041E-549.1811540
GTACGTA657.617382E-847.73317369
ACGAGAC2150.047.3978422
GGTATCA6900.047.244271
ACGCTGC2100.047.05622530
CGAGACT2150.045.82015623
ACTATAA306.8277E-445.2713283
TACGCTG2300.044.41102229
CTTATAC2550.043.944121
CGTATGC405.0232862E-543.09336541
TGCATCG1050.042.34219434
ATCTCCG2450.041.56960710
TCTCCGA2450.041.56960711
CCCACGA2400.041.0331619
CGCTGCA2300.040.0445831
GTTATTA700.001259482139.9715270
ATGCCGT459.7588105E-538.50221644
CACGAGA2600.037.88418221
Produced by FastQC (version 0.11.5)