FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1078487
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAATCTCCCCCTCACCTTCGGCGGAGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT43220.40074660148893776No Hit
TTATTATACTGTTGACAGGAATATGTTGCAATATCTTCAGGCTGCAGGCTGGTGATGGTGAAAGTAAAATCTGTC35700.33101928905958067No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33980.3150710207911639No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33650.3120111786233863No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG23410.21706334893234688No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22510.20871832483840788No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21590.20018785576460357No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18930.17552367344251715No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAAGACATTACCAACTATTTAAATTGGTATCAGCAGAAA18730.17366922364386403No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18290.1695894340868272No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18240.16912582163716391No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17860.165602367019723No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17090.15846273529490854No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16330.15141582606002668No Hit
AGTCAAGACATTACCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAG16030.14863415136204702No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15740.145945199154No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAAGACATTACCAA15480.14353441441575096No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15350.14232902204662645No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15110.1401036822882427No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA14980.13889828991911818No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14580.13518939032181196No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCTCCGCCGAAGGTG14370.13324221803322617No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14150.13120232325470776No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13750.12749342365740152No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13270.12304274414063406No Hit
ACATATTCCTGTCAACAGTATAATAATCTCCCCCTCACCTTCGGCGGAGGGACCAAGGTGGACATCAAACGAA12500.11590311241581958No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12380.11479044253662771No Hit
GTATATTACTGTGCGAAAGATAGGGGGGGAAGGAACTACTACTACGTGAT12370.11469772004669505No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12250.11358505016750317No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11910.1104324855097929No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11850.10987615057019694No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11300.10477641362390089No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCTGGTGCCAGATGTGACAT11170.10357102125477637No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11160.10347829876484371No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11090.10282924133531512No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC10970.10171657145612324No Hit
GGAATATGTTGCAATATCTTCAGGCTGCAGGCTGGTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA10870.1007893465567967No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)