FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1078487
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAATCTCCCCCTCACCTTCGGCGGAGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT45710.423834501482169No Hit
TTATTATACTGTTGACAGGAATATGTTGCAATATCTTCAGGCTGCAGGCTGGTGATGGTGAAAGTAAAATCTGTC36660.3399206480931157No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35180.3261977195830826No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33370.30941494890527194No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26740.24793993807992123No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23090.21409622925450192No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG22800.21140727704645487No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19740.18303419512706226No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18960.17580184091231513No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17990.16680775938884754No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17330.16068807505329225No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAAGACATTACCAACTATTTAAATTGGTATCAGCAGAAA17220.15966812766403304No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA16620.1541047782680737No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15810.1465942565835286No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15650.14511069674460608No Hit
AGTCAAGACATTACCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAG15380.1426071895164244No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14350.13305677305336086No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14270.1323149931338996No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14190.13157321321443838No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14180.1314804907245057No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAAGACATTACCAA14050.1302750983553812No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13730.1273079786775362No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCTCCGCCGAAGGTG13710.1271225336976709No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13640.12647347626814232No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13600.1261025863084117No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13570.12582441883861373No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12540.11627400237555019No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12420.11516133249635833No Hit
ACATATTCCTGTCAACAGTATAATAATCTCCCCCTCACCTTCGGCGGAGGGACCAAGGTGGACATCAAACGAA11970.11098882044938882No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11960.11089609795945617No Hit
GTATATTACTGTGCGAAAGATAGGGGGGGAAGGAACTACTACTACGTGAT11460.10625997346282338No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11120.1031074088051131No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA10900.10106751402659465No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10800.1001402891272681No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAA700.00130357939.69318470
CCGTTTA450.001304610239.6894269
GTATCAA34750.037.7666471
TATCAAC38950.033.3296172
GTGGTAT3350.032.477391
ATCAACG40900.031.569983
TCAACGC41350.031.1399064
AACGCAG42150.030.7906886
CAACGCA41850.030.7678665
TCTGGAG9750.029.92255470
ACCACGG7250.029.70144870
TACGTAT950.005824827629.24760870
ACGCAGA44400.029.2303497
CGCAGAG44750.028.9258128
AGAGTAC45700.028.77056311
GCAGAGT45950.028.3922189
CAGAGTA46600.028.14200210
CGTGTTC6850.027.159752
ACACCGT550.00902594426.7399266
TTAGTCG550.00902594426.7399266
Produced by FastQC (version 0.11.5)