FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1593368
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT43040.27011964593238974No Hit
GTTCTGGGGTGTCTCAACCATGGCCAGGACCCCTCTCTGGCTCACTCTCC41920.2630905101646324No Hit
CCCCTGTACTTGTCGTCCATGGTAAGAACACCCGGCCCTCAGGGATCCCA37890.23779817342886264No Hit
GTACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAAGTTGCATAATAGCTT37530.2355388083606549No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC37130.23302840272931302No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC34870.2188446109122312No Hit
GTCTATTATTGTAGCTCCCAGGACATCAGTGGTAACAATCTAGACTTCGG34820.21853081020831344No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAG34520.21664800598480702No Hit
GTAATATAGGGCCGTGTCGTCGGATGTCAGTCTGCTCAATCCCAAGTAGACTGTACTGATGGACACGTCCCTGGT34390.2158321241546209No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC32990.2070457044449242No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC32910.2065436233186558No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG31900.2002048490995175No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31700.19894964628384654No Hit
GACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGTCTAGATTGTTACCA30750.1929874329094095No Hit
AGCCAGGACAGGCCCCTGTACTTGTCGTCCATGGTAAGAACACCCGGCCC30200.1895356251663144No Hit
ATAATAGACAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG28670.17993332362643155No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC28430.1784270802476264No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGTCTAGATTGTTACCACTGATGTCCTGGGAG26910.16888753884852714No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26300.16505917026073072No Hit
ACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGTCTAGATTGTTACCAC24380.15300922323028954No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAG24320.15263266238558826No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG24180.1517540204146186No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA24030.15081261830286538No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT22590.1417751580300345No Hit
ATATTACTGTGCGAGAGATCTGTCCAATAGCAGTAGCTGGTACTTTGACTACTGGGGCCAGGGAGCCCTGGTCAC22320.1400806342288787No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGTAAGACTTCTGGATACACGTTCAGCGA22240.1395785531026103No Hit
GTCCTGGCTTCTGCTGGTACCAAGTTGCATAATAGCTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT21970.13788402930145452No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG21670.1360012250779481No Hit
GTCTCAACCATGGCCAGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAG21390.13424394113600877No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21240.13330253902425554No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG21050.13211009634936813No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT20030.12570856198944627No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19730.12382575776593982No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG19710.12370023748437273No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19430.1219429535424334No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19140.12012290945971049No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18880.11849114579933827No Hit
GCCCTATATTACTGTGCGAGAGATCTGTCCAATAGCAGTAGCTGGTACTT18750.11767526396915214No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGTCTAGATTGTTAC18580.11660834157583182No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG18170.11403517580370637No Hit
CTATATTACTGTGCGAGAGATCTGTCCAATAGCAGTAGCTGGTACTTTGA17570.1102695673566935No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17490.10976748623042511No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGCTATTATGCAACTTGGTA17370.10901436454102253No Hit
CAGTAATATAGGGCCGTGTCGTCGGATGTCAGTCTGCTCAATCCCAAGTA17060.10706880017673256No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17010.10675499947281483No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC16590.1041190735599058No Hit
GTCTAGATTGTTACCACTGATGTCCTGGGAGCTACAATAATAGACAGCCT16130.1012321070838626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13450.041.9488561
AATGGTA6700.040.85988670
GTATCAA34100.037.4772531
GTGGTAT4050.035.247521
TACGTAT1051.9235227E-434.30591670
CGAGTTT651.5428663E-533.80724366
TATCAAC38250.033.0442432
ATCAACG39000.032.4128463
TCAACGC39100.032.1490674
CAACGCA39750.031.623365
TGGTATC4650.031.419542
AACGCAG40550.031.0832546
ACGCAGA42900.029.380567
CGCAGAG42950.029.3454328
AGAGTAC43900.029.01994111
GACAGGT2100.027.5146451
ATACCGT500.00833823527.1789976
ATGGCAA5450.026.4375970
AGTACGG32350.025.83231713
GAGTACG32700.025.55742812
Produced by FastQC (version 0.11.5)