FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25532
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC580.22716590944696852No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTG560.21933260222465922No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG460.18016606611311295No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGC440.17233275889080368No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCCGCAGCAGTGGCAGCATTGCCAG410.1605827980573398No Hit
GAGTGGGGCTGAGTCAGCATAAAATTGGCCCAAGAACCTGTGCAGTGAGCGAGGAGGGTGAGAAGTAGTGGAGCC360.14099953000156668No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGG330.1292495691681028No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCCGCAGCAGTGGCAGCATTGCCAGCAACTATGTG320.12533291555694814No Hit
TCTCTGGACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT290.11358295472348426No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG280.10966630111232961No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTGATTGTTGCTATCATAAGACTGACAGTAGTAGTCAG280.10966630111232961No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG270.10574964750117499No Hit
CTCCGACACAGAGTGGGGCTGAGTCAGCATAAAATTGGCCCAAGAACCTG260.10183299389002036No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCA260.10183299389002036No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)