FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1380688
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCCCATGTACACTTTTGGCCAGGGGACCAAGCTGG47410.34337953252291614No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45480.3294009942869062No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTACTGATGCTGAGAGTGAACT41470.30035750292607744No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40480.29318716465993766No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA33300.24118410531561077No Hit
GTCTGGGACAGAGTTCACTCTCAGCATCAGTAGCCTGCAGTCTGAAGATT27920.2022180246369926No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26940.19512011403010673No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26620.19280242893398072No Hit
GTCCCAGACCCACTGCCACTGAACCTGCCTGGGATACCAGTGGCCCTGGT25140.18208313536439805No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23960.17353667157243347No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT23540.17049470988376808No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23280.16861159074316573No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21480.15557461207745704No Hit
CTGGTATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT21290.15419848655163224No Hit
GAGTTCACTCTCAGCATCAGTAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGG21060.15253265038879166No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGTAAGTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT21010.152170512092522No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19970.14463803553011254No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19600.14195821213771684No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19340.1400750929971145No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18520.13413602493829163No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGTAAGTTAGCCTGGTA18100.13109406324962627No Hit
GGATACCAGTGGCCCTGGTGGATGCATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG17810.1289936611312621No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACATGGGAGGCCAGTTATTATA17770.12870395049424635No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17610.12754510794618334No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17470.12653112071662823No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA17450.12638626539812037No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACATGGGAGG17270.12508256753154948No Hit
GTAATAAATGGCTGTGTCGTCAGATCGCAGGCTCCTCAGTTCCATGTAGACTGTGGTTGCGGATGTGTCCGTGGT17220.1247204292352798No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC17150.12421343562050224No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17110.12392372498348649No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17060.1235615866872168No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16980.12298216541318531No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGCCTGGGATACCA16470.11928835479123451No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15890.11508755055450615No Hit
GGCCAAAAGTGTACATGGGAGGCCAGTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGG15670.11349414205091954No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTACTGATGCTGAGAGTGAACTCTGTCCCAGACCCACTGCCA15470.11204558886584079No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG15450.11190073354733292No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCTCCCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA15120.10951062079195299No Hit
CCCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC15020.10878634419941363No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14900.10791721228836637No Hit
GTCAGAGTGTTGGCAGTAAGTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG14820.10733779101433488No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14780.10704808037731914No Hit
AAGTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCACCAGGGCCA14250.10320941443686046No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14250.10320941443686046No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14180.10270242082208292No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14140.10241271018506715No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGT200.00774271551.0895228
CTAACGC409.17149E-750.976643
TCGTATG3050.042.9195440
CGTATGC2900.041.63395741
TTCGATC503.9173556E-641.28524439
ATGCCGT2950.041.10056344
GCCGTCT3000.040.53678546
GGTATCA13300.039.3575171
ACATGCG3300.037.130427
CCTAACG558.20222E-637.0725752
ATCTCGT2600.036.95870637
GTATCAA34500.036.847761
ACGAGAC3500.035.93290322
CCGTCTT3500.035.78891847
ATGCGCA3600.035.02230529
CACGAGA3600.034.933521
CATGCGC3700.034.05967728
CTTATAC4000.033.990581
ATCTCCG3950.033.550610
CCCACGA4000.033.13601319
Produced by FastQC (version 0.11.5)