FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1380688
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47090.34106184742679013No Hit
GTATAATAACTGGCCTCCCATGTACACTTTTGGCCAGGGGACCAAGCTGG46930.33990300487872716No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTACTGATGCTGAGAGTGAACT40890.2961566986893491No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34820.2521931095222092No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA34700.251323977611162No Hit
GTCTGGGACAGAGTTCACTCTCAGCATCAGTAGCCTGCAGTCTGAAGATT27430.19866906933354964No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27360.1981620757187721No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26550.19229543531920318No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25920.18773249278620513No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25830.18708064385291973No Hit
GTCCCAGACCCACTGCCACTGAACCTGCCTGGGATACCAGTGGCCCTGGT23380.1693358673357051No Hit
GAGTTCACTCTCAGCATCAGTAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGG22560.16339679927688225No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22260.16122396949926412No Hit
CTGGTATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT22180.16064454822523264No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT21790.15781986951432908No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGTAAGTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT21580.15629888866999642No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20320.14717300360400032No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20170.14608658871519126No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACATGGGAGGCCAGTTATTATA20030.14507260148563614No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19640.14224792277473258No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19220.13920596108606723No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18800.13616399939740187No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA17960.13008007602007116No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17680.12805210156096092No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17680.12805210156096092No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC17450.12638626539812037No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGTAAGTTAGCCTGGTA17300.12529985050931128No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGCCTGGGATACCA16810.12175089520586838No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16580.12008505904302783No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACATGGGAGG16430.11899864415421876No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16190.11726038033212428No Hit
GTAATAAATGGCTGTGTCGTCAGATCGCAGGCTCCTCAGTTCCATGTAGACTGTGGTTGCGGATGTGTCCGTGGT16060.1163188207618231No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15820.1145805569397286No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15750.11407356332495105No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15750.11407356332495105No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15580.11284229311763411No Hit
GGATACCAGTGGCCCTGGTGGATGCATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG15540.11255258248061836No Hit
GGCCAAAAGTGTACATGGGAGGCCAGTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGG15130.10958304845120692No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTACTGATGCTGAGAGTGAACTCTGTCCCAGACCCACTGCCA14720.10661351442179551No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGCCTCCCATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA14670.10625137612552582No Hit
GTCAGAGTGTTGGCAGTAAGTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG14070.10190571657028959No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14010.10147115061476597No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG13920.10081930168148052No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13830.10016745274819511No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGA405.4955348E-542.4434551
GGTATCA16400.040.1661
GTATCAA38000.035.116381
AATGGTA7100.034.66611570
TATCAAC41600.031.7394682
ATCAACG41250.031.6784843
TCAACGC41600.031.3246964
CCTAACG553.5838428E-430.8567832
CAACGCA42500.030.742315
AACGCAG42850.030.5704046
TACGTTG1204.092392E-430.1629270
ACGCAGA45150.028.7876177
CGCAGAG45300.028.6173788
AGAGTAC45800.028.3780311
GCAGAGT47000.027.4378729
AACTGTA2756.864866E-926.32400370
TGCACCA24650.026.1371270
ATGGCAA5100.025.54976770
GACAGGT2600.024.8130991
CAGAGTA53100.024.41369610
Produced by FastQC (version 0.11.5)