FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1179543
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAACCACTCACACTGCTGTCATAGGA44600.37811254019565205No Hit
ATCTTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG42420.3596308061681516No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38860.3294496258296645No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCGCAGAGGTCAACTTCAGGGAGA38220.3240237956564534No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAGTTTCCAGGAACAGC36230.307152854961625No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC33040.28010848269202565No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32190.2729023019932296No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32090.2720545160286653No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAACCACTCACA25060.2124551627197991No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24940.211437819562322No Hit
GTGTTAAGATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAACTGCTGGTA24620.20872490447571643No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22670.19219307816671372No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22320.18922582729073886No Hit
CACCTGCACTGTCCCTGGTGGCTCCCTCAGTAGTGGTACTTACTACTGGA21580.18295221115296348No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21120.17905239571596795No Hit
GATGTACACTGGTACCAGCAGTTTCCAGGAACAGCCCCCAAACTCCTCAT21120.17905239571596795No Hit
CCTCCAGGCTGAGGATGAGGCTTATTACTACTGCCAGTCCTATGACAGCA21090.1787980599265987No Hit
GTCCCTGGTGGCTCCCTCAGTAGTGGTACTTACTACTGGAGCTGGATCCG20790.17625470203290597No Hit
GGTTATGATGTACACTGGTACCAGCAGTTTCCAGGAACAGCCCCCAAACTCCTCATCTATCTTAACACCAATCGG19930.1689637427376535No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19760.16752250659789425No Hit
GGCCATCACTGACCTCCAGGCTGAGGATGAGGCTTATTACTACTGCCAGT19580.1659964918616786No Hit
GTAATAAGCCTCATCCTCAGCCTGGAGGTCAGTGATGGCCAGGGAGGCTG19550.16574215607230935No Hit
GTCTATTACTGTGCGAGAGCCGCCCCTATGGCTTATCCCCCCTTTGACTC19510.16540304168648365No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19420.16464003431837584No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG19410.16455525572191942No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG19070.161672783442401No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC19050.16150322624948815No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18980.1609097760742932No Hit
GTCATAGGACTGGCAGTAGTAATAAGCCTCATCCTCAGCCTGGAGGTCAG18750.15895986835579543No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18670.15828163958414404No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC17770.15065156590306586No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17650.14963422274558877No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT17490.14827776520228597No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17460.14802342941291668No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17220.14598874309796253No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16750.1420041490645106No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16580.14056291292475134No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16500.13988468415309996No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAACCACTCACAC16230.13759566204877652No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16120.13666309748775585No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG16000.13564575433027876No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAG15620.13242416766493464No Hit
GCCTGGAGGTCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC14920.12648966591298494No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14640.12411586521220506No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14600.12377675082637937No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAAC14220.12055516416103526No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14030.11894437082836319No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13840.11733357749569114No Hit
GTAGTGGTACTTACTACTGGAGCTGGATCCGGCAGCCCGCCGGGAAGGGACTGGAGTGGATTGGGCGAATCTTTC13810.11707924170632185No Hit
GTCCTATGACAGCAGTGTGAGTGGTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG13660.1158075627594755No Hit
GGCTTATTACTACTGCCAGTCCTATGACAGCAGTGTGAGTGGTTCGGTATTCGGCGGAGGGACCAAGCTGACCGT13570.11504455539136768No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG13460.11411199083034701No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13370.11334898346223919No Hit
GGATGAGGCTTATTACTACTGCCAGTCCTATGACAGCAGTGTGAGTGGTTCGGTATTCGGCGGAGGGACCAAG13000.1102121753933515No Hit
CAGTAATAGACGGCCGTGTCTGCGGCGGTCGCAGAGGTCAACTTCAGGGAGAACTGGTTCTTGGACGTCTCCA12920.1095339466217001No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12880.1091948322358744No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12430.10537979539533532No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAACCACTCA12410.10521023820242247No Hit
ATACCGAACCACTCACACTGCTGTCATAGGACTGGCAGTAGTAATAAGCC12390.10504068100950961No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGAATTCCCTGGCCCCAGGAGTCAAAGGGGGGATAAG12380.10495590241305318No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12380.10495590241305318No Hit
TCACTGACCTCCAGGCTGAGGATGAGGCTTATTACTACTGCCAGTCCTAT12190.10334510908038112No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG12070.10232776592290405No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12050.10215820872999119No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC11990.10164953715125266No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11890.10080175118668841No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT702.5688132E-761.2823770
GGTATCA10750.048.987611
CCATACT306.592959E-445.594534
ACGAGAC3000.039.88675722
CGAGACT2950.039.40720423
GTATCAA28300.039.3917351
CTACGCT3050.039.264528
ACTACGC3000.038.7701427
TATACAC3200.038.468753
TCGTATG451.0010422E-438.33680340
GTGGTAT2500.038.2994041
ATCGCGT451.01857055E-438.2239737
CACGAGA3150.037.98738521
TACGCTG3200.037.4414529
CGAGCCC3300.037.29512415
TGGTATC2650.036.1299822
CCCACGA3450.035.673619
TGCATCG1750.035.30315434
GACTACG3300.035.23960526
AGACTAC3300.035.23362725
Produced by FastQC (version 0.11.5)