FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1179543
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTTAACACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG42020.35623966230989457No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCAGTTTCCAGGAACAGC36940.3131721353100311No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36290.30766152654036355No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAACCACTCACACTGCTGTCATAGGA35800.30350737531399874No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCGCAGAGGTCAACTTCAGGGAGA35100.29757287356204903No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33180.2812953830424156No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG29740.2521315458614057No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28650.24289067884765542No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27760.23534538376303363No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC26220.22228947990874434No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22200.18820848413326177No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG21350.18100230343446572No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21270.18032407466281433No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC21180.1795610672947065No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20860.17684815220810093No Hit
GGTTATGATGTACACTGGTACCAGCAGTTTCCAGGAACAGCCCCCAAACTCCTCATCTATCTTAACACCAATCGG20770.17608514483999313No Hit
GTGTTAAGATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAACTGCTGGTA20620.17481346589314675No Hit
GTCCCTGGTGGCTCCCTCAGTAGTGGTACTTACTACTGGAGCTGGATCCG20230.1715071006313462No Hit
GATGTACACTGGTACCAGCAGTTTCCAGGAACAGCCCCCAAACTCCTCAT20200.17125276484197693No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20040.16989630729867414No Hit
CACCTGCACTGTCCCTGGTGGCTCCCTCAGTAGTGGTACTTACTACTGGA19930.1689637427376535No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19630.16642038484396077No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT19230.1630292409857038No Hit
GTCTATTACTGTGCGAGAGCCGCCCCTATGGCTTATCCCCCCTTTGACTC19190.1626901265998781No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG18910.16031632589909822No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAACCACTCACA18880.16006199010972894No Hit
GGCCATCACTGACCTCCAGGCTGAGGATGAGGCTTATTACTACTGCCAGT18530.1570947392337541No Hit
CCTCCAGGCTGAGGATGAGGCTTATTACTACTGCCAGTCCTATGACAGCA18520.15700996063729766No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18410.156077396076277No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC18350.15556872449753847No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC18070.1531949237967586No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17450.14793865081646027No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17210.1459039645015061No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17140.14531051432631112No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16450.13946079117081786No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAG16240.13768044064523294No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG16190.13725654766295084No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT15190.12877868801730838No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15180.12869390942085196No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14920.12648966591298494No Hit
GTCATAGGACTGGCAGTAGTAATAAGCCTCATCCTCAGCCTGGAGGTCAG14730.12487887258031288No Hit
GGCTTATTACTACTGCCAGTCCTATGACAGCAGTGTGAGTGGTTCGGTATTCGGCGGAGGGACCAAGCTGACCGT14590.12369197222992295No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14320.1214029501255995No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14280.12106383573977379No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14170.12013127117875312No Hit
GTAATAAGCCTCATCCTCAGCCTGGAGGTCAGTGATGGCCAGGGAGGCTG14140.11987693538938386No Hit
GTCCTATGACAGCAGTGTGAGTGGTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG13670.11589234135593192No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13640.11563800556656265No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13520.11462066240908554No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC13440.11394243363743416No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG13330.11300986907641349No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13320.11292509047995707No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT12870.10911005363941798No Hit
GGATGAGGCTTATTACTACTGCCAGTCCTATGACAGCAGTGTGAGTGGTTCGGTATTCGGCGGAGGGACCAAG12850.10894049644650512No Hit
GGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGC12840.10885571785004872No Hit
GTAGTGGTACTTACTACTGGAGCTGGATCCGGCAGCCCGCCGGGAAGGGACTGGAGTGGATTGGGCGAATCTTTC12790.10843182486776658No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12520.10614280276344314No Hit
CAGTAATAGACGGCCGTGTCTGCGGCGGTCGCAGAGGTCAACTTCAGGGAGAACTGGTTCTTGGACGTCTCCA12410.10521023820242247No Hit
GCCTGGAGGTCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC12300.1042776736414018No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12240.10376900206266325No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAAC12100.1025821017122733No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12080.10241254451936047No Hit
TCACTGACCTCCAGGCTGAGGATGAGGCTTATTACTACTGCCAGTCCTAT12050.10215820872999119No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC11880.10071697259023199No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11800.10003874381858059No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAAAC251.9094594E-458.6072267
AGCCGAT200.007092851752.2344650
CGACGAA200.00744091751.60542338
CTAGACG200.007631154351.27696623
TTCGTAG200.007631154351.27696623
GGTATCA11150.046.910071
TGGTATC3100.042.98462
GTGGTAT3100.041.9055371
CGCGGTA602.8461363E-740.3650943
TTAACGG557.6422275E-637.4560435
GTATCAA27250.036.1306271
GCGATCT501.6451477E-435.23263558
GTCACCG10550.034.90081870
TAGGTTT900.003359832332.7292170
TAACGGC652.3892711E-531.71249636
TATCAAC30750.031.6672042
CGCGCTA450.00454329230.77550144
TGCGGTA450.00471881530.53795836
CCGTTAA450.004846147330.37217516
ATCAACG32200.030.2347853
Produced by FastQC (version 0.11.5)