FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443392
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTTTGGTAGCACTCCATTCACTTTCGGCCCTGGGA89900.6228384250432315No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT62930.43598689753026204No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGCGAGAGTGAAGTCTGTCCCAGACCCACTGCCA58490.40522602314547956No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53720.37217886755642265No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43310.30005708774885825No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA41160.2851616192967676No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38560.2671484946570301No Hit
GTGTATTACTGTCAGCAGTTTGGTAGCACTCCATTCACTTTCGGCCCTGGGACCAAACTGGATATCAAACGAA38540.26700993215980134No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37810.26195240101095196No Hit
GTCTGGGACAGACTTCACTCTCGCCATCAGCAGACTGGAGCCTGAAGATT36410.25225302620493945No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34740.24068305768633885No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32170.22287777679244444No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC31690.21955227685895445No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAACAGCTACTACTTAGCCTGGTACC31320.2169888706602226No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31150.21581108943377822No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30040.20812087083758257No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGCGAGAGTGAAGTCTGTCCCAGACCCACTGCCA29980.20770518334589633No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAACAG29820.20659668336806633No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCGGTGGCCCTGCT29120.20174699596506007No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28050.19433390236332196No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27790.19253258989934818No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27460.19024630869507383No Hit
GTGTTAACAGCTACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATGTATGGTGGAT26880.1862279962754401No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26770.18546590254068196No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC26710.18505021504899571No Hit
GTGCAGCCACAGTTCGTTTGATATCCAGTTTGGTCCCAGGGCCGAAAGTG26380.1827639338447213No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24990.1731338402873232No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT24480.16960049660799006No Hit
CCTCATGTATGGTGGATCCAGCAGGGCCACCGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT23160.16045537179089256No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22930.15886190307276193No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22720.15740699685186005No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCAGTTTGGTCC22140.1533886844322263No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA22020.15255730944885382No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21830.1512409657251807No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21080.14604487207910258No Hit
GTTTGGTAGCACTCCATTCACTTTCGGCCCTGGGACCAAACTGGATATCAAACGAACTGTGGCTGCACCAT20070.13904746596905068No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19810.13724615350507693No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19310.13378209107435818No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18990.13156509111869819No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18680.12941737241165255No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC18420.1276160599476788No Hit
GACTTCACTCTCGCCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTTTGGTAGCACT17880.12387487252250254No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17610.12200427880991443No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG17420.1206879350862413No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17280.11971799760564004No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT17110.11854021637919566No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT17100.1184709351305813No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16940.11736243515275128No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16570.11479902895401942No Hit
CTACCAAACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGCGAGAGTGAAGTCTGTC16560.11472974770540503No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT16130.11175065401498692No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15850.10981077905378442No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15740.10904868531902628No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15370.10648527912029442No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15330.10620815412583692No Hit
CATGTATGGTGGATCCAGCAGGGCCACCGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA15320.10613887287722254No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15290.10593102913137942No Hit
CTGCTACTCTGGCTCCCAGATACCACCGCAGAAATTGTGTTGACGCAGTC15260.1057231853855363No Hit
ATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCGC15220.10544606039107879No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGCAGAAATTGTGTTGACGCAGTCTC15170.10509965414800691No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14980.10378331042433378No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14810.10260552919788941No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14790.10246696670066066No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14600.10115062297698753No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG252.6238247E-454.9575936
ATCGCGT200.007462760451.56776437
CGTATGC552.562956E-950.2598241
TCGTATG605.6079443E-946.00523840
GGTATCA15250.042.908181
CGAGACG2100.040.59478423
AACTGTG33050.038.56594570
GTATCAA43950.037.764071
GAGACGC2300.037.0712324
ATCTCCG2500.036.7995210
CGCTCAT2300.035.6415228
ACGAGAC2450.034.78949722
CGCGTAC400.002755113234.0854420
CCCACGA2500.034.08189419
TATCAAC49000.033.6610642
TCTCCGA2650.033.43073311
TCAACGC50950.032.707194
ATCAACG50900.032.6723673
CAACGCA51750.032.3991285
AACGCAG52100.032.1160666
Produced by FastQC (version 0.11.5)