FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443392
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTTTGGTAGCACTCCATTCACTTTCGGCCCTGGGA82370.5706696448366071No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65000.4503281159934377No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGCGAGAGTGAAGTCTGTCCCAGACCCACTGCCA57040.3951802420963952No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42550.2947917128541657No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42280.2929211191415776No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41210.2855080255398395No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA39870.27622433822551323No Hit
GTGTATTACTGTCAGCAGTTTGGTAGCACTCCATTCACTTTCGGCCCTGGGACCAAACTGGATATCAAACGAA39520.2737994945240101No Hit
GTCTGGGACAGACTTCACTCTCGCCATCAGCAGACTGGAGCCTGAAGATT36580.25343080743138385No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34900.24179155766416885No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34800.2410987451780251No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC30620.21213918325721634No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGCGAGAGTGAAGTCTGTCCCAGACCCACTGCCA30250.20957577705848443No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAACAGCTACTACTTAGCCTGGTACC30200.2092293708154126No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29220.20243980845120382No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCGGTGGCCCTGCT28980.20077705848445884No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28170.19516527734669445No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27660.19163193366736134No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAACAG27500.19052343368953134No Hit
GTGTTAACAGCTACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATGTATGGTGGAT26920.18650512126989757No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26740.18525805879483884No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26620.18442668381146632No Hit
GTGCAGCCACAGTTCGTTTGATATCCAGTTTGGTCCCAGGGCCGAAAGTG26450.18324890258502194No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25110.1739652152706957No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24760.17154037156919258No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24710.1711939653261207No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC24510.1698083403538332No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23660.16391943422161132No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT23640.16378087172438258No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22890.15858477807830443No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22860.1583769343324613No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22350.1548435906531282No Hit
CCTCATGTATGGTGGATCCAGCAGGGCCACCGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT21910.15179521571409568No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA21790.15096384073072316No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21510.14902396576952068No Hit
GTTTGGTAGCACTCCATTCACTTTCGGCCCTGGGACCAAACTGGATATCAAACGAACTGTGGCTGCACCAT20550.14237296590254067No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCAGTTTGGTCC20410.14140302842193941No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20310.1407102159357957No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18640.12914024741719504No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18540.12844743493105132No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18530.12837815368243694No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC18070.12519121624617569No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17930.12422127876557441No Hit
GACTTCACTCTCGCCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTTTGGTAGCACT17780.1231820600363588No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17770.12311277878774443No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG17740.1229049350419013No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17450.12089577883208442No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT17080.11833237263335254No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT17060.1181938101361238No Hit
CTGCTACTCTGGCTCCCAGATACCACCGCAGAAATTGTGTTGACGCAGTC16750.11604609142907817No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16450.11396765397064693No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16060.1112656852746863No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15910.11022646654547066No Hit
CTACCAAACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGCGAGAGTGAAGTCTGTC15650.10842515408149693No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15530.10759377909812441No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15300.1060003103799938No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGCAGAAATTGTGTTGACGCAGTCTC14980.10378331042433378No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14530.10066565423668691No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14500.10045781049084379No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)