FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004692737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1582457
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC49650.31375260117652487No Hit
GCCTTGGGCTGACCTAGGACGGTCAACGTGGTCCCTCCGCCGAATACCCCAACACTCAGGCTGGTATCCCAAGTT45840.28967611758170997No Hit
GACCTAGGACGGTCAACGTGGTCCCTCCGCCGAATACCCCAACACTCAGG45840.28967611758170997No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG39970.25258190269940983No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39700.2508756952005647No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG38250.24171272900306295No Hit
GTCCTGGTACCGACAGGTCCCAGGAACAGCCCCCAAAGTCGTCATTTATG36930.23337127011982No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACGTGGTCCCTC32880.20777815763714275No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC32500.20537682856469403No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29980.18945222524213928No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28510.18016287330398234No Hit
CCCCAACACTCAGGCTGGTATCCCAAGTTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGG27140.1715054500691014No Hit
ACCTAGGACGGTCAACGTGGTCCCTCCGCCGAATACCCCAACACTCAGGC24840.15697108989375383No Hit
CCCTTAGACTCTCCTGTGTAGTCTCTGGATTCACTTTCAGTAATGCCTGG24620.15558084674654665No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG24290.15349548202573593No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23750.15008306702804564No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23690.14970390980608003No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23380.1477449308259245No Hit
GTGTTGGGGTATTCGGCGGAGGGACCACGTTGACCGTCCTAGGTCAGCCC23280.14711300212264852No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22800.1440797443469238No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC22790.1440165514765962No Hit
GGACAGAAGGTCACCATCTCCTGCTCAGGGAGCAGCTCCAACGTTGGGGA22640.14306865842168223No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21460.13561189972302565No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGGCCAGGGTGG21430.13542232111204286No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21260.13434804231647368No Hit
CTCCTGTGTAGTCTCTGGATTCACTTTCAGTAATGCCTGGATGACCTGGGTCCGCCAGGCTCCGGGAAAGGGG20330.12847110537600706No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG20310.12834471963535185No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19850.12543784760028234No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19830.12531146185962716No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19420.12272055417619564No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19420.12272055417619564No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19410.12265736130586803No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19210.12139350389931607No Hit
CTCCTGCTCAGGGAGCAGCTCCAACGTTGGGGAGAGGGGAGTGTCCTGGTACCGACAGGTCCCAGGAACAGCCCC18890.1193713320488329No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCAGG18530.1170963887170394No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18460.1166540386247462No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18320.11576933844015982No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT18210.11507421686655624No Hit
GATTATTACTGCGGAACTTGGGATACCAGCCTGAGTGTTGGGGTATTCGG17990.11368397371934909No Hit
GTCTGGAGTCCGGTGATGGCCAGGGTGGCTGACGTGCCAGACTTGGAGCC17920.11324162362705589No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC17890.1130520450160731No Hit
GTACTACTCAGATATAGGTCTGCGGTACAGTAATAAATGCCTGTGTCCTC16790.10610082928003732No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16670.10534251483610613No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16360.1033835358559506No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTG16330.10319395724496778No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16050.10142455687579505No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16000.10110859252415705No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)