FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1582457
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC48480.30635903534819586No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTG42010.26547324824623986No Hit
GCCTTGGGCTGACCTAGGACGGTCAACGTGGTCCCTCCGCCGAATACCCCAACACTCAGGCTGGTATCCCAAGTT40980.2589643826024972No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG40660.2569422107520141No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG38760.24493556538977046No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37280.23558302058128594No Hit
GACCTAGGACGGTCAACGTGGTCCCTCCGCCGAATACCCCAACACTCAGG33020.20866285782172914No Hit
GTCCTGGTACCGACAGGTCCCAGGAACAGCCCCCAAAGTCGTCATTTATG33000.20853647208107393No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32030.20240676365929688No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC30440.1923590972772088No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAC29500.18641896746641456No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28080.17744557987989562No Hit
GTGTTGGGGTATTCGGCGGAGGGACCACGTTGACCGTCCTAGGTCAGCCC26690.1686617709043595No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26230.16575489886928996No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACGTGGTCCCTC25740.16265844822323766No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG25190.15918284035521976No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG23350.1475553522149417No Hit
CCCTTAGACTCTCCTGTGTAGTCTCTGGATTCACTTTCAGTAATGCCTGG23080.14584914471609656No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22800.1440797443469238No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22710.1435110085139754No Hit
CCCCAACACTCAGGCTGGTATCCCAAGTTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGG22360.14129925805250948No Hit
CTCCTGTGTAGTCTCTGGATTCACTTTCAGTAATGCCTGGATGACCTGGGTCCGCCAGGCTCCGGGAAAGGGG21770.1375708787031812No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21330.13479039240876686No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20920.13219948472533535No Hit
GGACAGAAGGTCACCATCTCCTGCTCAGGGAGCAGCTCCAACGTTGGGGA20890.13200990611435254No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC20630.13036689148583502No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC20010.12644893352552392No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19900.12575381195192034No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19690.12442676167504077No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19520.12335248287947159No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18980.11994006788178131No Hit
ACCTAGGACGGTCAACGTGGTCCCTCCGCCGAATACCCCAACACTCAGGC18820.11892898195653974No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18790.11873940334555695No Hit
CTCCTGCTCAGGGAGCAGCTCCAACGTTGGGGAGAGGGGAGTGTCCTGGTACCGACAGGTCCCAGGAACAGCCCC18490.116843617235729No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18270.11545337408852183No Hit
GATTATTACTGCGGAACTTGGGATACCAGCCTGAGTGTTGGGGTATTCGG18240.11526379547753904No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT17840.1127360806644351No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17710.11191457335017635No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGGCCAGGGTGG17300.10932366566674481No Hit
GTCTGGAGTCCGGTGATGGCCAGGGTGGCTGACGTGCCAGACTTGGAGCC16400.10363630733726098No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16320.10313076437464018No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16210.10243564280103662No Hit
GTACTACTCAGATATAGGTCTGCGGTACAGTAATAAATGCCTGTGTCCTC16110.10180371409776064No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCAGG16090.10167732835710544No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCT16020.10123497826481226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15300.045.2233771
TACGTAT856.890231E-540.8437170
GTATCAA39800.037.7671241
GTGGTAT4650.035.184121
CCGTTAA807.9277015E-834.0684516
TATCAAC43850.033.8699342
AGGCTCG1052.3887133E-433.06395770
ATCAACG45150.033.037263
TCAACGC45000.032.9213834
CAACGCA45700.032.422245
AACGCAG46650.031.9090236
ATTAGAC450.003823793731.87961663
ACGCAGA49250.030.1553217
CGCAGAG49500.030.1406528
TCCTATA605.841882E-428.3867842
TATAAGA1850.027.6195762
GCCGTTA1005.609563E-727.25131615
AATGGTA6950.025.97542670
TTAACGG1209.0312824E-825.7204835
CTGTTCG801.0429502E-425.54739
Produced by FastQC (version 0.11.5)