FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1520901
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGTTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA89670.5895847264220354No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT75880.49891478801052797No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT70680.464724528420982No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56810.3735285860157893No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC54340.357288212710755No Hit
ATATTATGGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT51480.33848356993650475No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49810.32750323656832364No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC48100.3162599012032999No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAACTGTATAAAACACT47810.3143531367261906No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT45070.29633749994246833No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42560.2798341246405913No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA40950.2692482942676742No Hit
CCATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC40200.2643170068268743No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA39620.26050347787265576No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38110.2505751524918453No Hit
GTTTATTACTGTCAACAATATTATGGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA37220.2447233580620961No Hit
ATGGTACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA36200.23801680714260823No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35830.23558403867181363No Hit
GAGTACCATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAAT35100.23078425222943505No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34110.2242749528075792No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA34060.22394620031152587No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT33650.22125042984388857No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32740.21526713441571807No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32630.21454387892440074No Hit
GTCCCTGACCGCTTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC32250.21204535995439544No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31910.2098098429812328No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG30710.201919783075953No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC30250.19889526011226238No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC30250.19889526011226238No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30170.19836925611857706No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29770.19573923615015046No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG29530.19416122416909448No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA29400.19330646767935586No Hit
GGCCAAAAGTGTACGGAGTACCATAATATTGTTGACAGTAATAAACTGCC29390.19324071718014518No Hit
CACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAAC29120.19146545370145723No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27510.18087962332854013No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA27380.18002486683880148No Hit
GTCCCAGACCCGCTGCCACTGAAGCGGTCAGGGACCCCGGATTCCCGGGT27020.17765784886721753No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT26550.17456757540431628No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGTTCCAA26070.17141155144220432No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA25350.16667751549903642No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24600.16174622805823655No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24140.15872170509454595No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA23860.15688069111664732No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23550.1548424256411167No Hit
GATTTCACTCTCACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATGGTACT23310.15326441366006072No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC22830.15010838969794876No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22540.1482016252208395No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22230.14616335974530886No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGCTTCAGTGGCAGCGGGTCTGGGACAGATTT21740.14294158528398626No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21680.1425470822887223No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21460.14110057130608766No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20990.13801029784318639No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20790.13669528785897309No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAAGCGGTCAGGGACCCCG20600.13544602837397043No Hit
CTTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCACCAGCC20420.13426251938817846No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19840.13044899043395985No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19360.12729296647184793No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT19170.1260437069868453No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA19150.12591220598842395No Hit
TCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAAGCGGTCAGGGACC19040.12518895049710665No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18960.12466294650342133No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC18900.12426844350815734No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAACTG18780.12347943751762934No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18630.12249318002946936No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18580.12216442753341604No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18380.12084941754920274No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGTTCCAACAATAAGAACTA18260.12006041155867475No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17920.11782489458551215No Hit
GTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA17800.11703588859498415No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17750.11670713609893082No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17010.1118415991573416No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16940.11138134566286695No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATGGTACTCCGTACACTTTTGGCCAGGGGACCAAG16820.11059233967233896No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16770.11026358717628564No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT16710.10986908418102165No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16700.10980333368181099No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG16490.10842257319838701No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16370.10763356720785903No Hit
GTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAAGCGGTC16340.10743631571022702No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG16200.10651580872127772No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16160.10625280672443506No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATC15970.10500354723943241No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15800.1038857887528511No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15790.10382003825364045No Hit
GGATCTCTGGTGCCTCCGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG15740.10349128575758712No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15690.10316253326153378No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15660.1029652817639018No Hit
GTGTACGGAGTACCATAATATTGTTGACAGTAATAAACTGCCACATCTTC15520.10204477477495247No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15470.10171602227889914No Hit
GTACCATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAAT15400.1012557687844245No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15270.10040101229468584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14700.045.2488061
GTGGTAT3850.044.7267841
TGGTATC4000.043.0254632
GTATCAA36250.035.9531971
ATCAACG42800.030.430993
TATCAAC42700.030.2681792
GCTTTAC450.00513709830.0127411
TCAACGC43300.030.0026514
CAACGCA43750.029.8492895
AACGCAG44350.029.369386
GACAGGT1900.028.4331251
CACCGAC2850.028.4162968
GCACCGA2750.028.222557
CGACCGG2650.028.0132611
CCGTGTC7250.027.92452412
ACGCAGA47150.027.6968487
ACGAACC1403.6561687E-1027.69258552
AGAGTAC47550.027.46295511
CGCAGAG47400.027.4083888
ATACGGC7650.027.3483646
Produced by FastQC (version 0.11.5)