FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583525
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26540.4548219870613941No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18910.32406495008782826No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG17480.29955871642174714No Hit
GTAATATACGGCCGTGTCCTCGCCTCTCAGGCTGTTCATTTGCAGATACA15330.26271367979092586No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA13040.2234694314725162No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12780.21901375262413778No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT11960.20496122702540592No Hit
GTATATTACTGTGCGAAAGACTACCAAAATTACTATGAAAGTAGTGGTCATTACTCCCCTTTTGACTACTGGGGC11820.20256201533781756No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11580.19844908101623754No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT10850.1859389057880982No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCT10820.1854247889979007No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10730.18388243862730816No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10570.18114048241292147No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10230.17531382545734972No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10160.17411421961355555No Hit
CTCCTGTGCAGCCTCTACATTCACCTTTAGCAGTTATTCTATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG9610.16468874512660125No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAA9020.1545777815860503No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8920.15286405895205862No Hit
CTACGTAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAACTCCAAGAACACGCTGTATCTGCAAATGAA8730.1496079859474744No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC8370.14343858446510432No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8070.13829741656312927No Hit
GCCTAGAGCCTGAAGATTTTGGAGTTTATTACTGTCAGCAGCGTAACAACTGGCCGATCACCTTCGGCCAAGGGA7970.13658369392913755No Hit
GCGTAACAACTGGCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT7780.13332762092455336No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT7650.13109978150036417No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7630.13075703697356583No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT7630.13075703697356583No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7590.13007154791996914No Hit
GTCTACGTAGTATGTGCTCCCACCACTACTACTAATACTTGAGACCCATT7570.1297288033931708No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGGAGTTTATTACTGTCAGCAGCGTAACAACTGG7550.12938605886637247No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7430.12732959170558245No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAACTACTTGGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT7330.12561586907159078No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7310.12527312454479245No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7290.1249303800179941No Hit
GTCTTTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAACTACTTGGCCTGGTA7220.12373077417419989No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7150.12253116833040573No Hit
GCCCTGGGATTTGCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGA6750.11567627779443897No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6540.11207746026305643No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6520.1117347157362581No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG6450.1105351098924639No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA6400.10967824857546805No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC6390.10950687631206889No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC6250.10710766462448053No Hit
GTTGTTACGCTGCTGACAGTAATAAACTCCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGT6210.10642217557088386No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6210.10642217557088386No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT6150.10539394199048885No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC6100.104537080673493No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6090.10436570841009384No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6030.10333747482969881No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT6020.10316610256629964No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT5910.10128100766890878No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTCAT306.7758595E-445.3380733
GCGGTTC452.1020169E-645.18924331
CTGACGG307.154384E-444.840629
AATGGTA2350.043.868370
TCTGGAG7500.042.2180170
TTCTTAA709.775241E-442.07775570
GGCGACA350.001217398740.249650
TTATGGG350.001233064940.14509649
CAACCGA350.001304701839.68671844
GTATCAA12700.037.893361
GGTATCA4900.037.0875741
GGTAATC400.00197648536.46895261
GTGAGCG3050.036.21446270
TTTTATC400.00231178835.3230451
AGGCGAC400.002375798835.12695749
AATATAC7600.034.9579853
TAGTCAC400.002689496634.2499338
ATAGTCA400.002707968234.2021637
ACTAAGT400.002749897334.09515835
CCGTGTA400.002943749333.624712
Produced by FastQC (version 0.11.5)