FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441269
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGACGCTCCTTCGGCGGAGGGACCAAGGTGG34650.24041313592396701No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32520.22563449293643312No Hit
GTAATATACGGCCGAGTCCTCGGCTCTCAGACTGTTCATTTGCAAATACA32480.22535695973478928No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32000.2220265613150633No Hit
CAGTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGACTGAAGTCTGTCCCAGACCCACTGCCA26970.1871267612083518No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24750.1717236685171193No Hit
GTATATTACTGTGCGAAAGATGGCGATTTTTGGAGGGCTTACTATAAGGC23790.1650628716776674No Hit
GTGTATTACTGTCAGCAGTATGGACGCTCCTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCA22590.1567368756283525No Hit
CTCCTGTAGGGCCAGTCAGAGTATTAGCAGCAACTACTTAGCCTGGTACC22180.15389216031150327No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20960.14542739766136648No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA20040.13904413402355842No Hit
GTCTGGGACAGACTTCAGTCTCACCATCAGCAGACTGGAGCCTGAAGATT19940.13835030101944884No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19370.13439545289602425No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19200.13321593678903798No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18390.12759588945575046No Hit
AACATACACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAACTCCAAGAACACGCTGTATTTG18030.12509809064095598No Hit
GTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGACTGAAGTCTGTCCCAGACCCACTGCCA17940.12447364093725738No Hit
GTATGGACGTCTGGGGCCAGGGGACCACGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCG17550.12176769222123004No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17450.12107385921712047No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16740.1161476448879425No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16490.11441306237766856No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16010.11108266395794261No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGACCCTGCT15800.10962561464931253No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15760.10934808144766868No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15330.10636459952999752No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15230.10567076652588794No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15020.10421371721725786No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14590.10123023529958668No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14520.10074455219670998No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)