FastQCFastQC Report
Wed 8 May 2024
EGAF00004692748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441269
Total Bases106 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGACGCTCCTTCGGCGGAGGGACCAAGGTGG40450.28065545016232224No Hit
GTAATATACGGCCGAGTCCTCGGCTCTCAGACTGTTCATTTGCAAATACA38500.26712570658218554No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT37360.2592160103353364No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34350.23833163691163828No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31050.21543514777602238No Hit
CAGTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGACT29470.2044725863110911No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26790.18587786180095459No Hit
GTGTATTACTGTCAGCAGTATGGACGCTCCTTCGGCGGAGGGACCAAGGT26640.18483711229479022No Hit
GTATATTACTGTGCGAAAGATGGCGATTTTTGGAGGGCTTACTATAAGGC25220.17498468363643427No Hit
CTCCTGTAGGGCCAGTCAGAGTATTAGCAGCAACTACTTAGCCTGGTACC24850.17241750152122887No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC23160.1606917237517771No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22940.15916529114273603No Hit
GTCTGGGACAGACTTCAGTCTCACCATCAGCAGACTGGAGCCTGAAGATT22650.15715317543081828No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT21650.15021484538972255No Hit
GTATGGACGTCTGGGGCCAGGGGACCACGGTCACCGTCTCCTCAGCATCC21360.14820272967780476No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20830.14452541475602404No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20420.14168069943917477No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20260.14057056663259948No Hit
GTAATACACTGCAGAATCTTCAGGCTCCAGTCTGCTGATGGTGAGACTGA19540.13557496900301053No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19480.1351586692005448No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19120.13266087038575033No Hit
AACATACACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGA18960.13155073757917501No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA18830.13064875467383258No Hit
GAGTATTAGCAGCAACTACTTAGCCTGGTACCAGCAGAAACTTGGCCAGG17740.12308597492903822No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGC17630.1223227586245177No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGACCCTGCT17370.1205187928138328No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17210.1194086600072575No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17010.11802099399903834No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG16560.11489874548054527No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC16500.11448244567807953No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA16400.11378861267396995No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16120.11184588026246314No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15980.11087451405670974No Hit
GTATGGACGCTCCTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTG15950.11066636415547687No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15920.11045821425424401No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15920.11045821425424401No Hit
GGACTGAACAGAGAGAACTCACCATGCAGTTTGGGCTGAGTTGGCTTTTT15840.10990314785095634No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA15730.10913993154643582No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15530.10775226553821668No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC15340.10643398283040849No Hit
CGTCCATACCGTACGCCTTATAGTAAGCCCTCCAAAAATCGCCATCTTTC14990.10400556731602498No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAGGAG14570.10109146869876477No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC14500.10060578559588806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)