FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981824
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23480.23914673098233494No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22550.22967456489146731No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18210.18547112313408515No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17570.1789526432435956No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17490.17813783325728438No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17200.17518414705690633No Hit
GTATACTAGCTCACCGTACACTTTCGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT16450.1675453034352389No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14080.1434065575907698No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13670.13923065641092497No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGCTACCA13220.1346473502379245No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13110.13352698650674663No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13070.13311958151359102No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12950.13189736653412423No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12850.13087885405123525No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12670.12904553158203505No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT12490.1272122091128349No Hit
ATATTACTGTCAGCAGTATACTAGCTCACCGTACACTTTCGGCCAGGGGA12030.12252705169154554No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT11890.12110113421550095No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11770.11987891923603416No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11120.11325858809725572No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC11050.11254562935923342No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10870.11071230689003325No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10850.11050860439345545No Hit
CTCCTGCAGGGCCAGTGAGAGTCTTACCACCAATTACTTAGCCTGGTACC10600.10796232318623297No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10560.10755491819307737No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG10460.10653640571018838No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10290.1048049344892771No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT10150.1033790170132325No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG9940.10124014079916563No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG9880.10062903330943225No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGT200.00605743954.35972659
CCTAACG452.2354488E-644.7916682
GGTATCA12250.043.0702131
CTAACGC551.814933E-742.7447973
GTATCAA32250.038.1386031
GTGGTAT3700.033.605741
TATCAAC38600.032.0272032
AATGGTA4350.031.66299270
ATCAACG38700.031.5023943
TCAACGC38700.031.3256364
CAACGCA39850.030.3389075
TCGGTTG703.2443342E-530.32267451
AACGCAG40200.030.1598426
TGGTATC4200.029.5944962
AGAGTAC41900.029.25530211
CGCAGAG41800.029.0053988
CGGCAAA1000.00607446528.99663570
ACGCAGA42050.028.912827
AGCCTAT606.3445355E-427.9876636
CAGTTCG8750.027.33968770
Produced by FastQC (version 0.11.5)