FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1574903
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA70350.44669417735568473No Hit
GTATTACTGTCAGCAGTATGGTGGCTCAGTTCGGACGTTCGGCCAAGGGA48860.31024132914852537No Hit
GTCTATTACTGTGCGAGAGATTGTGGAAGCGACGACTTTTGGAGTGGTGGACTTGACTACTGGGGCCACGGAATC42770.27157228095952574No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG39030.24782478666940125No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG35660.22642664341867405No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34870.2214104614696905No Hit
CAGTAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA32420.2058539478304378No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28510.18102702198167125No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA26990.17137563392793081No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT23990.15232684171660096No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23820.15124741015795892No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGTCA23470.14902505106663713No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAGCTTC23270.1477551315858818No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21770.13823073548021686No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAG21620.13727829586965037No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC21490.13645284820715942No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21260.1349924408042908No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT21100.13397650521968654No Hit
GTGTATTACTGTCAGCAGTATGGTGGCTCAGTTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA20910.132770081712969No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC18530.11765803989198065No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATT18490.11740405599582958No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC18080.11480072106028118No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17950.1139752733977902No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17820.11314982573529925No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT17720.1125148659949216No Hit
GTATTAGGAACCGCTATGTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCAT17550.11143543443627958No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16580.10527632495461625No Hit
CTCCTGTGCAGCCTCTGGATTCAGCTTCAGAACCTATACCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG16360.10387941352578539No Hit
CAGTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA16290.10343494170752103No Hit
GTAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16170.10267299001906784No Hit
GTCTCCAGGCACCCTGACTTTGTCTCCAGGGGAAGGAGTCACCCTCTCCTGCAGGACCAGTCAGAGTATTAGGAA16090.10216502222676573No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16010.10165705443446359No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16000.10159355846042581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG401.3742465E-859.3218152
CTAACGC453.479363E-852.7321743
GGTATCA14500.046.994371
TCTGGAG20300.046.0697570
GTATCAA39000.038.334671
CGTATTG400.00275648434.0821632
CTTATAC3350.033.395421
TATCAAC44950.033.2571682
ACGAGAC3100.032.80990222
AATAGAC20650.032.5038873
GCGTCGT20500.032.4526962
GTCGTCT20500.032.3287964
TCAACGC46250.032.32344
ATCAACG46300.032.2884943
CAGCCGT20350.032.144039
AGCCGTG20350.032.14301310
GGCGTCG20650.032.14292561
TACGGGC2750.032.04511615
CGGCTCT20800.031.94657521
CGTCTCT19700.031.93845666
Produced by FastQC (version 0.11.5)