FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1574903
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA63150.40097707604849314No Hit
GTATTACTGTCAGCAGTATGGTGGCTCAGTTCGGACGTTCGGCCAAGGGA46510.2953197752496503No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG43880.2786203340777178No Hit
GTCTATTACTGTGCGAGAGATTGTGGAAGCGACGACTTTTGGAGTGGTGGACTTGACTACTGGGGCCACGGAATC41260.2619843888798231No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35800.22731558705520277No Hit
CAGTAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA32650.20731435523330644No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA31790.20185370146605855No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG30390.19296426510077128No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29600.18794808315178776No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGTCA23780.15099342626180787No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22710.14419935703976688No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22520.14299293353304934No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAG21800.13842122340233018No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21690.13772276768791475No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT21440.1361353683369706No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC21420.13600837638889507No Hit
GTGTATTACTGTCAGCAGTATGGTGGCTCAGTTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA21210.13467496093410197No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAGCTTC20510.13023024275145834No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19970.12680146015341898No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19780.1255950366467014No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAGGATT19170.12172178223039769No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC18210.11562616872277214No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC17550.11143543443627958No Hit
GTATTAGGAACCGCTATGTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCAT17440.11073697872186414No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT17080.10845112365650456No Hit
GAGTATTAGGAACCGCTATGTAGCCTGGTACCAGCAGAAACCTGGCCAGG16990.10787965989016467No Hit
GTAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16960.10768917196805138No Hit
CTCCTGTGCAGCCTCTGGATTCAGCTTCAGAACCTATACCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG16660.10578429274691838No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT16360.10387941352578539No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC16300.1034984376815588No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC15810.10038713495370825No Hit
GTCTCCAGGCACCCTGACTTTGTCTCCAGGGGAAGGAGTCACCCTCTCCTGCAGGACCAGTCAGAGTATTAGGAA15770.10013315105755718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG19700.048.50443670
GGTATCA14750.044.571091
TACGTAT1151.6082595E-743.4053470
CTAACGC551.7025013E-743.0970733
AACTGTG21000.038.71047670
GTATCAA38800.038.6041561
GTCGCTA500.002221644435.61389569
CGGCTCT20000.033.88566221
CCTAACG701.1264001E-633.8641362
AATAGAC20500.033.861983
CGTCGTC18900.033.810263
GTCGTCT19100.033.7935564
GCGTCGT19350.033.65756262
TCGGCTC20150.033.62806320
ATTTGCA19800.033.59721438
GGCGTCG19350.033.57439861
ATAGACA20800.033.371474
TAGACAG20700.033.369115
TATCAAC44600.033.3326342
GCAGATA20250.032.93974342
Produced by FastQC (version 0.11.5)