FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930165
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18850.20265221761730448No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCACGTGTTGGTTTTTGTATATGAGCTGCAGAAATAAT18810.2022221863862863No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17900.1924389758806233No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17890.1923314680728688No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC17380.18684856987738735No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15160.16298183655587986No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15050.16179925067057996No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14770.15878903205345288No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14060.15115597770288067No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13830.1486832981245263No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC13620.14642563416168097No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTTCTGCAGCTCATAT13230.14223282965925402No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCACGTGTTGGTTTTTGTATATGAGCTG13030.14008267350416323No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13020.1399751656964087No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13020.1399751656964087No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT12980.13954513446539055No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12330.13255712696134556No Hit
GTCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCAC11500.1236339789177189No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG11310.12159133057038267No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11180.12019372906957368No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11020.11847360414550107No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG10920.1173985260679557No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10870.11686098702918299No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10720.11524836991286493No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG10550.11342073718103778No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG10250.11019550294840164No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10020.10772282337004725No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAATGACGTT9600.10320749544435665No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC9530.10245494079007487No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC9320.10019727682722956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT353.374971E-758.9157639
TCGTATG1650.050.10997440
CGTATGC1950.049.5492141
GATCTCG1700.048.27279736
ATCTCGT1650.045.6631937
TGCCGTC2100.044.75095445
GCCGTCT1950.044.72525446
GGTATCA12300.043.576051
ATCGCGT504.0409795E-641.09686737
ATGCCGT2200.041.059544
CCGTCTT2150.040.63258447
GTATCAA32450.038.020891
AAAACCG1305.500624E-737.1026970
ACGAGAC3750.036.85998522
CGAGACA3800.036.3808523
CACGAGA3850.035.9025821
GTATGCC2700.035.87833842
AGGGGGG3700.035.38364870
AATCGGA400.002645494234.36752739
TCCGAGC3850.034.1438913
Produced by FastQC (version 0.11.5)