FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195802
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43820.3664486261103427No Hit
GATATATAGTGCTTATAGTTTGCTAACTGGGGTCCCATCAAGGTTCAGCGGCAGTCAATTTGGGACAGAGTTCA40580.33935383951523745No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39810.3329146464046724No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34700.2901818193981947No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31310.2618326445347976No Hit
CTATATATCAGGAGGTTAGGGGCTTCCCCTGGTCTTTGCTGATACCAGGC29980.25071040188927596No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28680.2398390368974128No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28340.23699575682261778No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG26610.22252847879498444No Hit
GTTTATTACTGTCAAGAGGTTACTGCCAACCCTCGAGCGCTCGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAA25570.21383138680149388No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25430.21266062441775477No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24600.20571967599987287No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24390.2039635324242642No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTCGGAGACACAGTCACCATCACTTGCCGGGCCAGTCAGGGCGTCAGCA23500.19652082869906556No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23400.19568456985353763No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22440.18765648493646941No Hit
GAGTTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAGTTTATTACTGTCAAGAGGTTACTGCCAAC22190.18556583782264957No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22090.18472957897712164No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21980.1838096942470409No Hit
GTAATAAACTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAACTCTGTCCCAAATTGACTGCCG21870.18288980951696016No Hit
CCTGATATATAGTGCTTATAGTTTGCTAACTGGGGTCCCATCAAGGTTCAGCGGCAGTCAATTTGGGACAGAGTT21090.17636699052184224No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC20940.17511260225355035No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20880.17461084694623358No Hit
GACCAGGGGAAGCCCCTAACCTCCTGATATATAGTGCTTATAGTTTGCTA20670.1728547033706249No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC20600.17226932217875535No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20360.1702623009494883No Hit
CTATAAGCACTATATATCAGGAGGTTAGGGGCTTCCCCTGGTCTTTGCTGATACCAGGCTAAATAAGTGCTGACG20200.1689242867966436No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19630.16415761137713433No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18920.15822017357388596No Hit
ATTTGGGACAGAGTTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATT18880.1578856700356748No Hit
GTGCTTATAGTTTGCTAACTGGGGTCCCATCAAGGTTCAGCGGCAGTCAA18160.15186460634787363No Hit
ATATATCAGGAGGTTAGGGGCTTCCCCTGGTCTTTGCTGATACCAGGCTAAATAAGTGCTGACGCCCTGACTGGC17860.14935582981128984No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17460.14601079442917808No Hit
GTGTCATCCAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTCGGAGACACAGTCACCATCACTTGCCGGG17210.14392014731535824No Hit
GTCAAGAGGTTACTGCCAACCCTCGAGCGCTCGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGG17210.14392014731535824No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16890.14124411900966882No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGCTGTGTCAT16880.14116049312511603No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16430.13739732832024032No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16210.13555755886007884No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15960.133466911746259No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGCGAGCGCTCGAGGGTTGGCAGTAACCT15820.13229614936251988No Hit
GGTTAGGGGCTTCCCCTGGTCTTTGCTGATACCAGGCTAAATAAGTGCTG15400.12878386221130256No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15230.12736222217390503No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15190.12702771863569387No Hit
CCTCTTGACAGTAATAAACTGCAAAATCTTCAGGCTGCAGGCTGCTGATT14970.1251879491755324No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14630.12234466910073741No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGCTGTGTCATCCAGTTGACCCAGTCTCCATCCTTC14470.12100665494789271No Hit
CTTTATTACTGTGCGAGAGCCCTCGACACCTGGGACTGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCA14110.11799612310399213No Hit
GTAATAAAGAGCCGTGTCCTCGCCTCTCAGGTTCTTCATTTGCAGATACA14010.1171598642584642No Hit
TATTTAGCCTGGTATCAGCAAAGACCAGGGGAAGCCCCTAACCTCCTGATATATAGTGCTTATAGTTTGCTAA13780.11523646891374993No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13610.11381482887635244No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13580.11356395122269407No Hit
CCCCTGGTCTTTGCTGATACCAGGCTAAATAAGTGCTGACGCCCTGACTGGCCCGGCAAGTGATGGTGACTGTGT13530.11314582179993007No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAG13420.11222593706984936No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13340.11155692999342699No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13250.11080429703245187No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13080.10938265699505435No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12900.10787739107310408No Hit
GTCAGGGCGTCAGCACTTATTTAGCCTGGTATCAGCAAAGACCAGGGGAAGCCCCTAACCTCCTGATATATAGTG12890.10779376518855127No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12830.10729200988123452No Hit
CTCGAGGGTTGGCAGTAACCTCTTGACAGTAATAAACTGCAAAATCTTCA12800.10704113222757615No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12750.10662300280481216No Hit
ACCCTCGAGCGCTCGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA12700.10620487338204819No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12620.10553586630562585No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12480.10436510392188673No Hit
CCCCTAACCTCCTGATATATAGTGCTTATAGTTTGCTAACTGGGGTCCCA12470.10428147803733395No Hit
GTCGGAGACACAGTCACCATCACTTGCCGGGCCAGTCAGGGCGTCAGCACTTATTTAGCCTGGTATCAGCAAAGA12420.10386334861456996No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12260.10252533446172528No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTACG300.002327964969.2010170
GAGCGTA200.007090824752.2382747
CGCGTAT408.324987E-751.696439
GGTCGAC200.00776353551.0544137
ATCGCGT507.17464E-848.10130337
ACGAGAC3850.041.58381322
CTACGCT3700.041.504828
CCTAACG603.2324533E-739.715632
GTATCAA34000.039.3534431
CGAGACT4000.039.17611323
TACGCTG3950.038.90074529
CAACCGT350.001440538638.8985989
ATCTCCG4350.038.3397110
GCATCGC901.3096724E-1038.10005635
CCCACGA4300.038.00351319
CTTATAC4200.037.288741
CACGAGA4500.037.0818821
ACGCTGC4250.036.97841330
TATACAC4650.036.6027343
GTGGTAT2450.036.1307031
Produced by FastQC (version 0.11.5)