FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195802
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44140.3691246544160321No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43850.36669950376400107No Hit
GATATATAGTGCTTATAGTTTGCTAACTGGGGTCCCATCAAGGTTCAGCGGCAGTCAATTTGGGACAGAGTTCA34610.28942918643721954No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33830.2829063674421016No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32720.2736238942567415No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31400.2625852774957727No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG27050.2262080177153074No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27000.2257898882925434No Hit
CTATATATCAGGAGGTTAGGGGCTTCCCCTGGTCTTTGCTGATACCAGGC26990.2257062624079906No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26590.22236122702587888No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24780.20722494192182317No Hit
GTTTATTACTGTCAAGAGGTTACTGCCAACCCTCGAGCGCTCGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAA24710.20663956072995363No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23370.19543369219987924No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTCGGAGACACAGTCACCATCACTTGCCGGGCCAGTCAGGGCGTCAGCA22840.19100152031858117No Hit
GTAATAAACTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAACTCTGTCCCAAATTGACTGCCG22780.19049976501126442No Hit
GAGTTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAGTTTATTACTGTCAAGAGGTTACTGCCAAC22760.19033251324215883No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22740.19016526147305324No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21300.17812313409745092No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21230.17753775290558135No Hit
GTGCTTATAGTTTGCTAACTGGGGTCCCATCAAGGTTCAGCGGCAGTCAA20740.17344008456249446No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20690.17302195513973048No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20230.16917516445030198No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20050.16766989852835168No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19680.1645757407998983No Hit
GACCAGGGGAAGCCCCTAACCTCCTGATATATAGTGCTTATAGTTTGCTA18860.1577184182665692No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18790.15713303707469967No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC18290.15295174284705998No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18270.1527844910779544No Hit
CCTGATATATAGTGCTTATAGTTTGCTAACTGGGGTCCCATCAAGGTTCAGCGGCAGTCAATTTGGGACAGAGTT18230.1524499875397432No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18100.15136285104055688No Hit
CTATAAGCACTATATATCAGGAGGTTAGGGGCTTCCCCTGGTCTTTGCTGATACCAGGCTAAATAAGTGCTGACG17890.14960670746494822No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17680.14785056388933954No Hit
ATATATCAGGAGGTTAGGGGCTTCCCCTGGTCTTTGCTGATACCAGGCTAAATAAGTGCTGACGCCCTGACTGGC17520.14651254973649483No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17440.1458435426600725No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17100.14300026258527748No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17080.14283301081617192No Hit
ATTTGGGACAGAGTTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATT17040.14249850727796073No Hit
GTCAAGAGGTTACTGCCAACCCTCGAGCGCTCGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGG16960.1418295002015384No Hit
GTGTCATCCAGTTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTCGGAGACACAGTCACCATCACTTGCCGGG16940.1416622484324328No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGCTGTGTCAT16550.13840083893487384No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGCGAGCGCTCGAGGGTTGGCAGTAACCT15800.13212889759341429No Hit
CCTCTTGACAGTAATAAACTGCAAAATCTTCAGGCTGCAGGCTGCTGATT14670.12267917263894859No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14610.12217741733163183No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14530.12150841025520948No Hit
GGTTAGGGGCTTCCCCTGGTCTTTGCTGATACCAGGCTAAATAAGTGCTG14260.11925051137228404No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14170.11849787841130889No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13840.11573822422106671No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13810.1154873465674083No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13790.11532009479830271No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGCTGTGTCATCCAGTTGACCCAGTCTCCATCCTTC13500.1128949441462717No Hit
CCCCTGGTCTTTGCTGATACCAGGCTAAATAAGTGCTGACGCCCTGACTGGCCCGGCAAGTGATGGTGACTGTGT13170.1101352899560295No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12970.10846277226497364No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12720.10637212515115378No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAG12610.10545224042107304No Hit
GTAATAAAGAGCCGTGTCCTCGCCTCTCAGGTTCTTCATTTGCAGATACA12550.10495048511375629No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12340.10319434153814762No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12330.10311071565359484No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12240.10235808269261969No Hit
GTCGGAGACACAGTCACCATCACTTGCCGGGCCAGTCAGGGCGTCAGCACTTATTTAGCCTGGTATCAGCAAAGA12200.1020235791544085No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12170.10177270150075013No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12150.10160544973164454No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12020.10051831323245823No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG300.0020381371.5600270
GCGATTT451.2730174E-463.6089170
GTGGTAT3500.047.5758821
CGTAGCT350.001458299238.8017274
GTATCAA33850.037.948271
AATGGTA4500.034.984970
GAGTAGA1052.0003502E-434.07620270
CTAACGC502.045748E-433.950093
TATCAAC39200.032.484562
CCTAACG652.5873653E-531.3450982
ATCAACG40650.030.98523
TCAACGC41000.030.6391684
CAACGCA41850.030.0168695
AACGCAG42550.029.6826366
GTCGCTG3000.028.318981
TCTTCGG3301.8189894E-1228.19031370
CGCAGAG46200.027.1906038
CGACCGG3000.027.16120711
AGAGTAC46750.026.72546611
CAGAGTA47650.026.5056910
Produced by FastQC (version 0.11.5)